Inocula Preparation of Ectomycorrhizal Fungi and Their Effects on the Growth of Pine Seedlings

In the experiment, four species of ECM fungi, Lactarius insulsus Fr., Russula delica Fr., Cortinarius russus Fr. and Pisolithus tinctorius (Pers.) Coker et Couch, were isolated from the fruit bodies collected from different forest types. The species were cultured in different media and inoculated on the seedlings of Pinus tabulaeiormis Carr., P. massoniana Lamb., Larix gmelini (Rupr.) Rupr., P. sylvestris var. mongolica Litv., P. koraiensis Sieb. et Zucc., P. taeda L., P. elliottii Engelm.The results indicated that PDMA and PD were suitable media for the four ECM fungi. Saw-dust, wheat brans, rice brans, corn folur, vermicul-ate, peat,perlite were suitable solid media for the development of the ECM fungi.The four species of ECM fungi could enhance the growth of 5 pines. An excellent fungus for symbiont of the pines was C. russus, then L. insul-sus, R. delica, P. tinctorius successively. For P. taeda, the best one was L. insulus, successively R. delica, P. tinctorius. The seedling growth of P. elliottii is better than that of the controls. P. sylvestris var. mongolica possessed the strongest adaptibility to the infection of these mycorrhizal fungi,