Effects of Calcium Chloride and Magnesium Sulphate on the Propagation in Vitro of Chinese Fir

The effects of calcium chloride (CaCl22H2O) and magnesium sulphate (MgSO47H2O) on shoot multiplication in vitro of Cunninghamia lanceolata (Lamb.) Hook,the absorption of calcium and magnesium by the shoots in the cultures,and the amount of remains of calcium and magnesium in the media were studied to choose the concentration level of calcium chloride and magnesium sulphate suitable for mass-propagation in vitro of Chinese Fir.400 mg/L calcium chloride(CaCl22H2O) and 400 mg/L magnesium sulphate (MgSO47H2O) were recommended.It appears that for the tissue culture of Chinese Fir,the effect of calcium chloride is higher than that of magnesium sulphate.In addition,the results show that the high concentration of calcium chloride is favourable to the callus formation,but it is adverse to the differentiation.