A Study on Rattan Tissue Culture

This paper deals with tissue culture of three important commercial rattan species in China. The results show that the immature embryo is the best one of eight kinds of explants selected for rattan tissue culture. The embryoes cultured respectively in media RM5-1 and RM8-2 for 50 days were introduced into complete plantlets. Callus could be introduced from different explants shining 16 hours a day with 690 lx on the medium with salt concentration of 6899mg/L. RM6 is the super medium for introducing multiple shoots. It is estimated that 2.8×106 shoots can be obtained from one of the subcultured shoots a year. Microscopical observation on the multiple shoots shows that the distance between the nodes on a main shoot is very short, only 420 nm and the meristematic tissue of nodes can produce many axillary bud primordium while it is carrying out periclinal and anticlinal division. And the axillary bud primordium can be introduced into multiple shoots further. The meristematic cells of shoots from two years old seedlings can be rejuvenated by tissue culture, that is the cells, introduced by tissue culture, and have the following characters;smaller cell diameter, higher density and an enlarged nuclear.