Study on pollen viability of Clones from a Masson Pine Seed Orchard

Study on the pollen germination rate(PGR)of 27 clones from a clonal seed or chard of Pinus massoniana was reported in this paper. Seven solution media were used for the culture to evaluate the effects of different clones(A),incubating media(B)and their interaction (A×B) on pollen viability.In addition,the correlation between pollen size and PGR was analyzed.The results are as follows:(1)the influences of A,B and A×B on PGR were all at the level of extreme significance(P0.01);(2)there was isgnificant correlation between the pollen size and its germination rate,but no enough evidence has been found to regard pollen size as an equivalent parameter of PGR;(3)the pollens studied could be divided into three types batsed on their PGR through robust princiole component analysis;(4)the solutions containing 5% glucose and 30～50 ppm boric acid allowed the pollens to germinate best. Some problems on pollen viability test were also discussed with some suggestions.