Preliminary Comparision of Bursaphelenchus xylophilus and B. mucronatus Utilizing Enzyme Electrophoresis

The enzyme electrophoregrams of Bursaphelenchus xylophilus and B. mucronatus that were collected from China,Japan,Canada,France and Norway were analysed. The phenotypes of Malate dehydrogenase(MDH)and Cellulase vary among isolates. Super-oxide dismuatase(SOD)shows a similar pattern with just one main band which migratesslowly.Esterase(Est)is different between B. xylophilus and B. mucronatus but the pheno-type of an isolate from Pinus thunbergii in Nanjing,China shows great difference within theisolate after being cultured on Schizophyllum sp.,Pestalotis sp.,and Botrytis cineria,separately. Peroxidase and soluble proteins were also analysed but gave less clear results. Glutamate-oxaloacetate transaminase(GOT)isozyme patterns,however,can be used for differenting between B. xylophilus and B. mucronatus. Both nematodes have only one band whichis identical introspecies and different interspecies on the basis of Rf.This result coincideswith that of Kiyohara and Bolla's(1990).