Establishment of Saline-tolerant Cell Lines of Populus × xiaozhuanica cv.‘Popularis’-39 and the Induction of Somaclonal Variant Plant

Somaclonal variants of Populus × xiaozhuanica cv.'popularis'-39,which is tolerant to 30‰～3.5‰ NaCl,is successfully obtained through the establishment of NaCl-tolerant pressure cell culture to produce calli and control the conditions of adventitious bud and root induction. The friable calli are well produced on the medium M4 MS+0.45 mg/L 2,4-D+0.3mg/L NAA+0.1mg/L Kinetine.The best suspension liquid medium is LM3:MS+0.5mg/L2,4-D+30g/L sucrose.The addition of amino acids was beneficial for the growth of the suspension cells.The relative parameters about cell growth are also measured.The culture test of NaCl-tolerant cells indicates that NaCl restrains cell growth and the higher the concentration of NaCl,the stronger the restraint.Calli can be produced from all the cell lines tolerant to the every NaCl level from 3.0‰～6‰;The test,in which the shoots can be induced only from the calli tolerant to 3.0‰～4.0‰,indicates that the high concentration of NaCl exerts an obvious restraining impact on the shoot induction.The root differentiation is also highly sensible to NaCl and the roots can only be induced on the medium with 3.0‰ and 3.5‰ NaCl.