Technological Study on Introduction of Exogenous DNA into Pigeon Pea and Its Application in Breeding

An experiment has been made on the introduction of general DNA extracted from soy bean into 860 flowers of pigeon pea and 620 different individuals have thus been obtained. Single selection and succession breeding have been carried out and 13 family systems of progeny of the single plants developed with remarkable mutant characteristics. And according to the protein analysis of their seeds with SDS-PAGE, IEF,it has been found that five individual plants have shown the presence of a band spectrum of soy bean protein. The seeds of these different plants have been applied to establish D2 and D3 generation groups and their germination and survival rate have got up to 95% respectively. Finally,a varied material of pigeon pea with high protein contents has been obtained in the light of analysing the components and contents of the seeds of the plant of the D3 generation. It′s protein content is 30.88%,which is much more than that of the receptor,pigeon,by 66%. And the protein contents of the other four have reached above 20%. Through breeding for third generation of D3,it has shown that the features of these varied plants are relatively stable.