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Volume 10 Issue 5
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Article Navigation >  Forest Research  > 1997 >  10(5): 478-481

Citation:
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PCR Amplification of 16 S rDNA of Phytoplasma Associated with Cherry Fasciated Disease and RFLP Analysis

  • 1.

    Institute of Microbiology, Chinese Academy of Science Beijing 100080

  • 2.

    The Research Institute of Forest Protection, CAF

  • 3.

    Capital Airport Animal & Plant Quarantine Bureau, PRC

  • Received Date: 1996-12-30
  • Cherry fasciated disease is a disease which was found in the cherry sapling imported from Israel.DNA amplification by PCR was used to detect the phytoplasma associated with cherry fasciated disease.The 16 S rDNA sequence amplified was analyzed by restriction endonuclease digestion.The RFLP pattern(sum of results analyzed by 7 restriction enzymes)was compared with the results of other phytoplasma previously described by Lee,I.M.et al.We propose that cherry fasciated disease should belong to Group Ⅶ.
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  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

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PCR Amplification of 16 S rDNA of Phytoplasma Associated with Cherry Fasciated Disease and RFLP Analysis

  • 1. Institute of Microbiology, Chinese Academy of Science Beijing 100080
  • 2. The Research Institute of Forest Protection, CAF
  • 3. Capital Airport Animal & Plant Quarantine Bureau, PRC
  • Received Date: 1996-12-30
  • Available Online: 2012-03-17

Abstract: Cherry fasciated disease is a disease which was found in the cherry sapling imported from Israel.DNA amplification by PCR was used to detect the phytoplasma associated with cherry fasciated disease.The 16 S rDNA sequence amplified was analyzed by restriction endonuclease digestion.The RFLP pattern(sum of results analyzed by 7 restriction enzymes)was compared with the results of other phytoplasma previously described by Lee,I.M.et al.We propose that cherry fasciated disease should belong to Group Ⅶ.

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