Studies on the Relationship of Amino Acid Content of Different Masson Pine Provenances and Their Resistance to Pine Wood Nematode (PWN)

On the basis of selected and tested resistance of masson pine (Pinus massoniana) provenances, and external symptoms of resistant difference of masson pine provenances, GX2(H), SC2(M), SX1(L) and black pine(S) (P.thunbergii) resistant to PWN (Bursaphelenchus xylophilus) were observed.The resistant provenance GX2 was stable (IR=100.0).However, those of the other provenances SC2, SX1 and black pine varied (IR=60.0, 40.0 and 0 respectively).Comparing with SC2, SX1 and black pine, the resistant factors in wood of GX2 were analysed and the results showed that:the factor that less sucking stimulatory of free amino acid (including His, Thre, Gly, Ile, Glu, Cys, Meth, Leu, Tyr, Try and Arg) existed in the wood of GX2 indicated it seemed to be one of the anti feeding causes.A clear positive correlation was found between the extrernal symptoms of index of pathogenesis (IP) and the content of free amino acid.It indicated that the pines with low content of free amino acid are resistant to PWN and vice versa.And a negative correlation between the IP and the total crude protein and 11 stationary amino acids were also found.