Comparison of Antisenile Effects of Seed Oil of Camellia grijsii and Certain Other Oil from Woody Crops on 2BS Cell Culture

The seed oil of Camellia grijsii (GRI) and C.oleifera (OLF), olive oil (OLV) and olein (OLN) after enzymolisis etc.were added to MEM medium to culture the human diploid foetal lung fibroblasts(2BS). The results showed the similar antisenile effects of the seed oil of C.grijsii and olive oil. Significant lower accumulation of LPO in cell membrane tested at 46th generation of the above two groups than that of OLF,unsaponifiable fraction of GRI(NSG),OLN+NSG and OLN groups were found. The lifespan of cells in GRI and OLV groups wast 2 generations longer than that in OLF group. The comparison of morphology of 2BS under microscope revealed the same sequence as the result of LPO testing and lifespan observation. Preliminary conclusion is that an appropriate existence of linoleic acid is essential to prolong the lifespan of 2BS with the main component of oleic acid in certain edible oils,and there are some active components in unsaponifiable fraction of GRI which could delay the aging process of 2BS.