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Citation:

Study on Several Nitrogen Fixation Strains from Phyllostachys pubescens Roots

  • Received Date: 1997-09-02
  • Four strains GW-1,GW-5,GW-10 and GW-16 isolated from Phyllostachys pubescens roots are identified and studied. According to the research on morphology,physiological and biochemical character,these strains all belong to Bacillus polymyxa. Their optimum temperature for nitrogen fixing is 30~35 ℃ for GW-1,30 ℃ for GW-5 and GW-16,35 ℃ for GW-10. Optimum pH for nitrogen fixing is 7.5 for GW-1 and GW-10,7.0 for GW-5,6.0~7.5 for GW-16. Many carbon sources can be used by these four strains,but the highest nitrogenase activity can be obtained when the carbon sources is the mixture of glucose,sucrose and malic acid. Under different culture time condition the highest nitrogenase activity is 156.90,89.37 nmol (C2H4)·h-1 ·bottle -1 for GW-1 and GW-5 in 96 h,56.63,83.64 nmol (C2H4)·h-1 ·bottle -1 for GW-10 and GW-16 in 72 h.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Study on Several Nitrogen Fixation Strains from Phyllostachys pubescens Roots

  • 1. The Research Institute of Subtropical Forestry, CAF Fuyang, Zhejiang 311400

Abstract: Four strains GW-1,GW-5,GW-10 and GW-16 isolated from Phyllostachys pubescens roots are identified and studied. According to the research on morphology,physiological and biochemical character,these strains all belong to Bacillus polymyxa. Their optimum temperature for nitrogen fixing is 30~35 ℃ for GW-1,30 ℃ for GW-5 and GW-16,35 ℃ for GW-10. Optimum pH for nitrogen fixing is 7.5 for GW-1 and GW-10,7.0 for GW-5,6.0~7.5 for GW-16. Many carbon sources can be used by these four strains,but the highest nitrogenase activity can be obtained when the carbon sources is the mixture of glucose,sucrose and malic acid. Under different culture time condition the highest nitrogenase activity is 156.90,89.37 nmol (C2H4)·h-1 ·bottle -1 for GW-1 and GW-5 in 96 h,56.63,83.64 nmol (C2H4)·h-1 ·bottle -1 for GW-10 and GW-16 in 72 h.

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