A Studies on the Technology of Propagation of the Nuclear Polyhedrosis Virus of the Poplar Looper,Apocheima cineraius

This paper deals with the study on the main factors,such as the infection concentration,infection instar and temperature,affecting the propagation of the Nuclear Polyhedrosis Virus (NPV) of poplar looper, Apocheima cinerarius, the artificially reared 3～4 instars larvae were fed with the surface inoculated medium and it proved that the optimal concentration is 2.5×106PIB·mL-1 on condition that the temperature is 20 ℃.The result showed that the 4-instar larvae were the best for inoculation.The average propagating total of polyhedral inclusion bodies(PIB) is 2.40×109～2.43×109PIB.