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Volume 14 Issue 2
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Article Navigation >  Forest Research  > 2001 >  14(2): 131-140

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Study on the Relationship between the Changes of Activities and Isoenzymes of Oxidases and the Rooting of Eucalyptus Cuttings Treated with Indoleacetic Acid

  • 1.

    College of Biotechnology, South China Agricultural University, Guangzhou 510642, Guangdong, China

  • 2.

    College of Forestry, South China Agricultura University,Guangzhou 510642,Guangdong,China.

  • 3.

    Leizhou Forestry Institute of National Forestry Bureau,Zhanjiang 524348,Guangdong,China

  • Received Date: 2000-02-21
    Accepted Date: 2000-05-22
  • Eucalyptus urophylla MLA clone(MLA) was difficult to root species. E. urophylla U6 clone(U6) and E. ABL 12 W5 clone(W5) were relatively easy to root species. The activities of peroxidase(POD) and indoleacetic acid oxidase(IAAO) in MLA were higher than that in U6 and W5. But the activity of polyphenol oxidase(PPO) in MLA was lower than that in U6 and W5. After the cuttings of Eucalyptus were treated with indoleacetic acid(IAA), the activities of POD, PPO and IAAO and the content of protein increased regularly in different stages of rooting. The isoenzymes of POD and PPO increased after treatment with IAA. The relationship between the oxidases and rooting of Eucalyptus cuttings was discussed.
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Study on the Relationship between the Changes of Activities and Isoenzymes of Oxidases and the Rooting of Eucalyptus Cuttings Treated with Indoleacetic Acid

  • 1. College of Biotechnology, South China Agricultural University, Guangzhou 510642, Guangdong, China
  • 2. College of Forestry, South China Agricultura University,Guangzhou 510642,Guangdong,China.
  • 3. Leizhou Forestry Institute of National Forestry Bureau,Zhanjiang 524348,Guangdong,China
  • Received Date: 2000-02-21
  • Accepted Date: 2000-05-22

Abstract: Eucalyptus urophylla MLA clone(MLA) was difficult to root species. E. urophylla U6 clone(U6) and E. ABL 12 W5 clone(W5) were relatively easy to root species. The activities of peroxidase(POD) and indoleacetic acid oxidase(IAAO) in MLA were higher than that in U6 and W5. But the activity of polyphenol oxidase(PPO) in MLA was lower than that in U6 and W5. After the cuttings of Eucalyptus were treated with indoleacetic acid(IAA), the activities of POD, PPO and IAAO and the content of protein increased regularly in different stages of rooting. The isoenzymes of POD and PPO increased after treatment with IAA. The relationship between the oxidases and rooting of Eucalyptus cuttings was discussed.

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