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Volume 14 Issue 3
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Article Navigation >  Forest Research  > 2001 >  14(3): 251-257

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Study on Induction Embryogenic Callus of Larix principis-rupprechtii by 311-A Regression Design

  • 1.

    Research Institute of Forestry, CAF, Beijing 100091, China

  • 2.

    Research Centre of Forestry Biotechnology, Shanxi Agricultural University,Taigu 030801,Shanxi, China

  • 3.

    Federal Research Centre for Forestry,Institute for Forestry Tree Breeding and Biotechnology

  • 4.

    and Biotechnology,Eberswalder Chaussee 6,15377 Wald sieversdorf,Germany

  • Received Date: 2000-03-10
  • The effects of 2,4-D, BA and KT on induction embryogenic callus(EC) of L. principis-rupprechtii were carried out by 311-A regression design. The regression equation was established which expresses the height of induction EC of L. principis-rupprechtii as function, and the 2,4-D, BA and KT as variable respectively. Using the regression equation, the single factor effect and mutual effect between the height of induction EC of L. principis-rupprechtii and 2,4-D, BA and KT was studied; the optimum concentration recipes can be obtained by computer processing, that are 2,4-D: 1.29 mg· L-1 ,BA: 0.39 mg· L-1 and KT: 0.58 mg· L-1 , the target height of induction EC is 13.931 7 mg per explant. The results of experiment showed that this method is simple, practical and rapid for selecting several hormone category and concentration recipes media of conifer somatic embryogenesis.
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Study on Induction Embryogenic Callus of Larix principis-rupprechtii by 311-A Regression Design

  • 1. Research Institute of Forestry, CAF, Beijing 100091, China
  • 2. Research Centre of Forestry Biotechnology, Shanxi Agricultural University,Taigu 030801,Shanxi, China
  • 3. Federal Research Centre for Forestry,Institute for Forestry Tree Breeding and Biotechnology
  • 4. and Biotechnology,Eberswalder Chaussee 6,15377 Wald sieversdorf,Germany
  • Received Date: 2000-03-10

Abstract: The effects of 2,4-D, BA and KT on induction embryogenic callus(EC) of L. principis-rupprechtii were carried out by 311-A regression design. The regression equation was established which expresses the height of induction EC of L. principis-rupprechtii as function, and the 2,4-D, BA and KT as variable respectively. Using the regression equation, the single factor effect and mutual effect between the height of induction EC of L. principis-rupprechtii and 2,4-D, BA and KT was studied; the optimum concentration recipes can be obtained by computer processing, that are 2,4-D: 1.29 mg· L-1 ,BA: 0.39 mg· L-1 and KT: 0.58 mg· L-1 , the target height of induction EC is 13.931 7 mg per explant. The results of experiment showed that this method is simple, practical and rapid for selecting several hormone category and concentration recipes media of conifer somatic embryogenesis.

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