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Volume 15 Issue 6
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Article Navigation >  Forest Research  > 2002 >  15(6): 746-750

Citation:
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Microdissection and PCR Amplification of Single Cunninghamia lanceolata Chromosome

  • 1.

    College of Resources and Environment, Nanjing Forestry University, Jiangsu 210037, Nanjing,China

  • Received Date: 2001-09-17
  • The single chromosome of Cunninghamia lanceolata was successfully dissected by "glass needles" under the micromanipulator in this research. The dissected Cunninghamia lanceolata chromosomes were digested by proteinase K in Eppendorf respectively. After degenerate olionucleotide primed PCR amplification (DOP PCR), DNA fragments were acquired. The suitable method of making chromosome specimens for the microdissection and PCR amplification has been discussed.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Microdissection and PCR Amplification of Single Cunninghamia lanceolata Chromosome

  • 1. College of Resources and Environment, Nanjing Forestry University, Jiangsu 210037, Nanjing,China
  • Received Date: 2001-09-17

Abstract: The single chromosome of Cunninghamia lanceolata was successfully dissected by "glass needles" under the micromanipulator in this research. The dissected Cunninghamia lanceolata chromosomes were digested by proteinase K in Eppendorf respectively. After degenerate olionucleotide primed PCR amplification (DOP PCR), DNA fragments were acquired. The suitable method of making chromosome specimens for the microdissection and PCR amplification has been discussed.

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