Establishment of Efficient Agrobacterium tumefaciens-mediated Genetic Transformation System of Carnation(Dianthus caryophyllus L.)

Efficient genetic transformation system of five carnation (Dianthus caryophyllus) cultivars has been established and optimized by using leaf explant. The results show that 2 days preculture can increase the transformation frequency obviously. There are differences between carnation cultivars in transformation. 20μmol AgNO3in selection medium inhibits the adventitious shoot inducing. 72.1% transformed plants rooted on rooting medium containing 25 mg·L-1 kanamycin and 55% plants show GUS activity according to histochemical assay. PCR screening indicates that 32.2% of the samples are positive. Southern blot analysis demonstrates that foreign genes are integrated into plant genome.