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Volume 16 Issue 3
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Article Navigation >  Forest Research  > 2003 >  16(3): 372-375

Citation:
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RAPD Analysis on Male Sterility Mutant Initiated from Irradiation Lily

  • 1.

    Department of Landscape and Gardening, Beijing Agricultural College, Beijing102206, China

  • 2.

    Institute of Vegetable and Flowers, Chinese Academy of Agricultural Science, Beijing100081, China

  • 3.

    College of Landscape and Gardening, Beijing Forestry University, Beijing100083, China

  • Received Date: 2002-10-25
  • RAPD analysis on Lilium regale and its 13 phenotype male sterility mutants initiated from irradiation bulbs was made by means of 300 random 10 mer primers. Of the tested primers, 93 primers produced ideal amplification bands on all materials, 14 primers generated stable different polymorphic bands among mutant ‘Wang 2G03'mutant ‘Wang 3G03' and Lilium regale. 21 different polymorphic bands were found between ‘ Wang 2G03' and Lilium regale. 25 different polymorphic bands existed between ‘Wang 1G03' and Lilium regale. It was showed that ‘Wang 2G03' and ‘Wang 1G03' were two real different male sterility mutants of Lilium regale. From the 93 primers RAPD bands, the other 11 phenotype male sterility mutants showed no difference in comparison with Lilium regale. It was proposed that more test need to be made to identify whether they were real mutants.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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RAPD Analysis on Male Sterility Mutant Initiated from Irradiation Lily

  • 1. Department of Landscape and Gardening, Beijing Agricultural College, Beijing102206, China
  • 2. Institute of Vegetable and Flowers, Chinese Academy of Agricultural Science, Beijing100081, China
  • 3. College of Landscape and Gardening, Beijing Forestry University, Beijing100083, China
  • Received Date: 2002-10-25

Abstract: RAPD analysis on Lilium regale and its 13 phenotype male sterility mutants initiated from irradiation bulbs was made by means of 300 random 10 mer primers. Of the tested primers, 93 primers produced ideal amplification bands on all materials, 14 primers generated stable different polymorphic bands among mutant ‘Wang 2G03'mutant ‘Wang 3G03' and Lilium regale. 21 different polymorphic bands were found between ‘ Wang 2G03' and Lilium regale. 25 different polymorphic bands existed between ‘Wang 1G03' and Lilium regale. It was showed that ‘Wang 2G03' and ‘Wang 1G03' were two real different male sterility mutants of Lilium regale. From the 93 primers RAPD bands, the other 11 phenotype male sterility mutants showed no difference in comparison with Lilium regale. It was proposed that more test need to be made to identify whether they were real mutants.

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