Techniques of Tissue Culture and in vitro Clonal Propagation of Ilex latifolia

The optimum medium for in vitro growth and multiplication of Ilex latifolia from nodal segments was MS+1.0 μmol NAA+8.8 μmol BA or MS+1.0 μmol NAA+9.1 μmol ZT. When calli resulted from cultured young leaves were transferred to WPM supplemented with 5.0 μmol TDZ and 3.0 μmol IAA,the most of adventitious shoots (11.2 per callus) formed. And BA was suitable for the multiplication and height growth of adventitious shoots. 1/4 MS+1.0 μmol NAA(or+1.0 μmol IBA) optimized the rooting of shoots. And the plantlets got acclimatized well, with the survival up to 90%.