Study on the Optimization of RAPD Conditions of Dendrocalamus latiflorus Munro
- Received Date: 2002-12-19
Abstract: Based on the genomic DNA of Dendrocalamus latiflorus,some essential factors that might affect the results of RAPD were compared and the optimal RAPD reaction system for D.latiflorus was established.The optimized condition for 20 μL reaction system were 2 μL 10 diploid buffer, 50 ng template DNA, 2. 5 mmol·L-1 MgCl2,1. 5 U Taq DNA polymerase, 1. 75 mmol·L-1 dNTP and 0. 4 μmol·L-1 Random primer.The optimized reaction program was initially at 94 ℃ for 3 min, followed by 40 cycles at 94 ℃ for 1 min, at 37. 5 ℃ for 1 min, at 72 ℃ for 1 min 20 sec, and then held at 72 ℃ for 8 min,and finally kept at 4 ℃.