• 中国中文核心期刊
  • 中国科学引文数据库(CSCD)核心库来源期刊
  • 中国科技论文统计源期刊(CJCR)
  • 第二届国家期刊奖提名奖

Citation:

Establishment of Cell Suspension Culture Line and Suspension Culture from the Neem Tree

  • Received Date: 2002-10-25
  • Studied on the establishment of suspension culture line for the cell of the neem tree (Azadirachta indica) and suspension culture condition. The cell of neem' s suspension cultural line grew best in B5 liquid medium, contained with NAA 2.0~4.0 mg·L-1, inoculation with direct spill method. On the basis of integrative analysis for biomass of cell, content and productivity of azadirachtin, the optimum condition of suspension culture was B5 liquid medium, contained with NAA 2.0~4.0 mg·L-1, saccharose 30 g·L-1, temperature 25 ℃. And according to the size of cultural vessel to decide optimum rotate speed of shake and broth content.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article views(2785) PDF downloads(1331) Cited by()

Proportional views

Establishment of Cell Suspension Culture Line and Suspension Culture from the Neem Tree

  • 1. Research Institute of Forest Ecology, Environment and Protection, CAF, Beijing100091, China

Abstract: Studied on the establishment of suspension culture line for the cell of the neem tree (Azadirachta indica) and suspension culture condition. The cell of neem' s suspension cultural line grew best in B5 liquid medium, contained with NAA 2.0~4.0 mg·L-1, inoculation with direct spill method. On the basis of integrative analysis for biomass of cell, content and productivity of azadirachtin, the optimum condition of suspension culture was B5 liquid medium, contained with NAA 2.0~4.0 mg·L-1, saccharose 30 g·L-1, temperature 25 ℃. And according to the size of cultural vessel to decide optimum rotate speed of shake and broth content.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return