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Citation:

An AFLP Linkage Map Using a Hybrid Population of Populus tomentosa

  • Received Date: 2002-07-02
  • The map was constructed using Populus tomentosa × P.alba var. pyramidalis × P. tomentosa hybrid population in which the resistance of Dothrorella gregaria Sacc was segregated.The authors had obtained 662 markers using 19 PstI/Mse1 primer pairs and 4 EcoRI/Mse1 primer pairs,of which 43. 2% had 3∶1 in segregation,14. 5% appears to be non-parental heteroduplex,and 48. 8% was distorted.239 markers had been mapped into 22 linkage groups (each including 4 loci or more),and the integrated map was 4 418 cM in length,with an average locus interval of 18. 5 cM.Linkage group LG1 was the largest one and covered 524 cM with 25 markers.The densest linkage group was LG13 with an average interval of 6. 7 cM in length.There were duplicated loci for 8 markers,and 7 markers that linked to one of markers in a linkage group but couldn′t be mapped into that group.In addition,there were 15 minor groups harboring 2~3 markers belonged to the parents.Moreover,13 and 21 groups belonged to paternal and maternal parent,respectively.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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An AFLP Linkage Map Using a Hybrid Population of Populus tomentosa

  • 1. Research Institute of Forestry, CAF, Beijing100091, China
  • 2. Beijing Forestry University, Beijing100083, China
  • 3. Department of Statistics, 533 McCarty Hall C, University of Florida, Gainesville, FL 32611, USA

Abstract: The map was constructed using Populus tomentosa × P.alba var. pyramidalis × P. tomentosa hybrid population in which the resistance of Dothrorella gregaria Sacc was segregated.The authors had obtained 662 markers using 19 PstI/Mse1 primer pairs and 4 EcoRI/Mse1 primer pairs,of which 43. 2% had 3∶1 in segregation,14. 5% appears to be non-parental heteroduplex,and 48. 8% was distorted.239 markers had been mapped into 22 linkage groups (each including 4 loci or more),and the integrated map was 4 418 cM in length,with an average locus interval of 18. 5 cM.Linkage group LG1 was the largest one and covered 524 cM with 25 markers.The densest linkage group was LG13 with an average interval of 6. 7 cM in length.There were duplicated loci for 8 markers,and 7 markers that linked to one of markers in a linkage group but couldn′t be mapped into that group.In addition,there were 15 minor groups harboring 2~3 markers belonged to the parents.Moreover,13 and 21 groups belonged to paternal and maternal parent,respectively.

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