Construction of Repeated Carnation ACC Oxidase Gene PlantRecombinant Expression Vectors

The genomic fragment of carnation ACC oxidase,the key enzyme in ethylene biosynthesis,was cloned and single copy plant recombinant expression vectors were constructed first.The sense and antisense repeated plant recombinant expression vectors were constructed based on the single copy vectors.The repeated vectors were indentified by restriction enzyme and PCR analysis.The vectors were transformed into Agrobacterium tumefaciens,which was confirmed by PCR analysis.Transgenic plants of carnation were obtained by using those vectors,which were confirmed by PCR analysis and Southern blotting.The transgenic plants were under further analysis for transgene silence effect.