Study on Isolation Culture of Lactarius hatsutake and Its Effects on Growth of Masson Pine Nursery Stock

The results showed that PDA was a more suitable medium for isolation culture of Lactarius hatsutake.Through tissue separation, the pure mycelium could be obtained.When fruit bodies of L. hatsutake from different districts isolated at the same condition, the growth velocity of mycelium was different. Mycelia grew faster on PDA, MMN,Yeast-glucose -gallium,while slowly and weakly on the medium of MS, Cha, and peptone. Using liquid ferment Mycelia of L. hatsutake as inoculum to nursery stock of Masson Pine, mycorrhigal seedling could be formed. Formed mycorhiza could improve the growth of Masson Pine, and achieve the aim of taming culture, which will provide a new way for multi-management of forest production.