Study on Tissue Culture of Lilium japonicum cv.'Louvre'

Using bulb scale as the explant and MS as the basic media, the bulbous projection of Lilium japonicum cv.'Louvre'was induced and cultured with different hormones in differet concentration levels. The result revealed that the best way to induce 'Louvre'was to use its whole scale, whose back was stuck on the medium that contains 2.0 mg·L-1 of 6-BA, 0.2 mg·L-1 of NAA and MS as the explant.The induction of 'Louvre'scale was to produce the bulbous projection which differentiated into seedings, but not to produce it from callus induction. MS containg 0.7 mg·L-1 of 6-BA and 0.3 mg·L-1 of IBA was optimized as the best medium for shoot-tip culture and subculture, and 1/2MS containing 0.3 mg·L-1 of 6-BA and 0.7 mg·L-1 of IBA was optimized as the best medium for rooting culture. The material for transplanting was the combanation of river mud and pearly rock(cubic comparison is 7∶3). The survival rate of Lilium shoots could reach 98%.