DNA Molecular Evidences of the Inter-specific Hybrids between Paeonia ostii and P.suffruticosa Based on ISSR Markers

Paeonia ostii was believed to be one of the main progenitors of the Chinese tree peony cultivars,and hybridization was the most important pathway for incorporation of genetic composition of P.ostii.However,no experimental evidence had been reported till now.In this study,P.ostii was used as a maternal parent crossing with other two Chinese tree peony cultivars which were used as paternal parents.The relationships between the F1 hybrids and their parents were analyzed by means of DNA fingerprinting based on ISSR （Inter-simple sequence repeats） markers,and ISSR fragments typically inherited from both parents were detected in the genomes of the F1 generation.This study also indicated that ISSR marker was suitable for identification of F1 hybrids in P.suffruticosa at seedling stage.