Expressed Sequence Tag (EST) and Its Application in Forest Research
- Received Date: 2003-08-06
Abstract: This article has introduced the basic principles and procedure of EST analysis,and also reviewed the application of EST in novel gene finding,gene expression analysis and the use in preparation of gene-chips in genomic studies on different biological processes,wood formation for instance.EST database provide a resource to develop molecular markers such as SNP,SSR,etc.,the latter has been used to construct genetic linkage maps.It's prospect of its application on forest genomics is also discussed.