Genetic Diversity of Michelia chapensis Dandy Populations

Michelia chapensis,belonging to Michelia genus in the family Magnoliaceae, is a elite species for timber production and for landscaping. In this paper,the genetic diversity were investigated using RAPD technique.The total DNA were extracted following a modification of CTAB method with some adjustments. A total of 144 individuals representing 6 natural population of M. chapensis were surveyed using 19 RAPD primers.At species level,the percentage of polymorphic bands was 81.98%,Nei's gene diversity was 0.325 5 and Shannon index was 0.475 1.AMOVA showed that 29.96% of the genetic diversity resulted from differentiation among populations. The coefficient of gene differentiation(Gst)generated by POPGENE32 was 0.222 6. The 6 natural populations of M. chapensis might be divided into 2 groups used UPGMA cluster system,and the genetic diversity of second group was higher than that of the first.