Study on Organogenesis of Liriodendron chinense×L.tulipifera

The organogenesis systems of Liriodendron hybrids were established by using the leaves,petioles,stem fragments and basal shoot fragments as explants.The results indicated that the callus could be induced from these explants on the inductive media,and the basal shoot fragment had the highest callus-inducing rate.The calli differentiated into shoot bud on the differentiation media MS+6-BA 2.0 mg·L-1+NAA 0.5 mg·L-1 and MS+6-BA 4.0 mg·L-1+NAA 0.5 mg·L-1,and some explants directly differentiated into shoot on the medium MS+6-BA 4.0 mg·L-1+NAA 0.5 mg·L-1.The organogensis systems offered base for the molecular breeding.