Several Factors Affecting Accumulation of Flavonoids in Suspension-cultured Cells of Ginkgo biloba
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1.
Research Institute of Subtropical Forestry, CAF, Fuyang 311400, Zhejiang, China
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2.
Research Institute of Forestry, CAF, Beijing 100091, China
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Received Date:
1999-10-22
Accepted Date:
2005-09-06
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Abstract
The cell suspension culture system was established for producing flavoniods by using stem of young seedling as ex-plants. A study was made on the phase of cell growth and relative changing amount of flavonoids, as well as the activity of L-phenylalanine ammonia-lyase (PAL) (EC 4. 3.1.5). It was showed that the flavonoids accumulation increased in the exponential phase of cell growth, and reached the highest on the 15th day after culture. The activity of PAL increased during this period. PAL activity doubled in response to transferring suspension-cultured cells into new medium. In cell suspension cultures, growth in the dark, but not in light-growth cultures, PAL activity was induced up to 12 times by UV-light.
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Proportional views
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