Study on Callus Induction and Brown Stain Prevention Techniques of Some Sympodial Bamboo Species

The stem tops and stems with node of young bamboo of Bambusa ventricosa,B. multiplex cv. Fernleaf and B. multiplex were used to study the callus induction and brown stain control. The results showed that among three regulator combinations(2,4-D KT and IBA) ,2,4-D(2-3 mg·L-1)treatment was the best with the highest callus induction rate,most of the explants induced callus and grew well. 2,4-D(2 mg·L-1) + KT(0. 5 mg·L-1) treatment could induce callus from stem parts while the callus induced from the stem top was water-soaked and easy to be brown stained. KT(4 mg·L-1) treatment could induce callus on some stems but was easy to be brown stained. KT(4 mg·L-1) + IBA(0. 5 mg·L-1) treatment could induce callus but they grew very slowly. The effect of adding anti-oxidant was better than adding adsorbent in controlling brown stain of callus. The ability of brown stain control is in the order of ascorbic acid(5 mg·L-1) > cyste-ine(100 mg·L-1) > activated carbon(1 g·L-1) . By adding ascorbic acid(5 mg·L-1) in culture media,the brown stain rates of stem top and middle part of Bambusa ventricosa, B. multiplex, var. nana and B. multiplex reduced by 51. 3% , 43. 3% ,36. 8% and 62. 7% ,42. 7% ,30. 8% respectively compared with that of the control. Soaking explants in non-bacteria water or cysteine(100 mg·L-1)for 2 to 4 hours would benefit controlling the brown stain. Culturing in dark would benefit the growth of callus and control of brown stain.