Analysis of Relationships among the Main Commercial Species of Lac Insects Using Random Amplified Polymorphic DNA (RAPD)

CHEN Hang; CHEN Xiao-ming; FENG Ying; YE Shou-de

The technique of random amplified polymorphic DNA(RAPD)was used to study the relationships of 12 populations from 7 species in the genus Kerria.A total of 288 samples were used in this study and 24 samples for each population.In 45 primers under predetermined optimal reaction conditions,9 primers were informative and yielded a total of 121 clear and reproducible loci.The percentage of polymorphic loci(PPB) was 97.52%.The amplified DNA loci of individual primer were 11～16,and their molecular size was 150～3 000 bp.The dendrogram were constructed based on Nei's genetic distance by UPGMA method.The cluster results showed the genetic distance between inter-population were 0.044 3～0.791 7,in which the average genetic distance among species was 0.443 0 while under species was 0.170 7.By analysis of molecular dendrogram,the genetic relationships among production species were discussed.

1. Research Institute of Resource Insects, CAF, Kunming 650224, Yunnan, China

2. The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2006-03-10

Abstract: The technique of random amplified polymorphic DNA(RAPD)was used to study the relationships of 12 populations from 7 species in the genus Kerria.A total of 288 samples were used in this study and 24 samples for each population.In 45 primers under predetermined optimal reaction conditions,9 primers were informative and yielded a total of 121 clear and reproducible loci.The percentage of polymorphic loci(PPB) was 97.52%.The amplified DNA loci of individual primer were 11～16,and their molecular size was 150～3 000 bp.The dendrogram were constructed based on Nei's genetic distance by UPGMA method.The cluster results showed the genetic distance between inter-population were 0.044 3～0.791 7,in which the average genetic distance among species was 0.443 0 while under species was 0.170 7.By analysis of molecular dendrogram,the genetic relationships among production species were discussed.

HTML

Reference (23)

[1]	陈晓鸣. 紫胶虫生物多样性研究[M]. 昆明: 云南科技出版社, 2005
[2]	Varshney R K. Taxonomic studies on lac insects of India (Homopetra: Tachardiidae) [J]. Orient Insects (supp lement) , 1976 (5) : 1～97
[3]	欧炳荣,洪广基. 紫胶虫外部形态扫描电镜观察[J]. 林业科学研究, 1990, 3 (2) : 133～136
[4]	欧炳荣,洪广基. 云南紫胶虫外部形态扫描电镜观察[J]. 林业科学研究, 1991, 4 (3) : 269～272
[5]	陈晓鸣,王绍云,毛玉芬,等. 四种紫胶虫雄性外生殖器观察及初步杂交试验[J]. 林业科学研究, 1992, 5 (2) : 236～238
[6]	陈晓鸣,王绍云,毛玉芬,等. 紫胶虫杂交试验[J]. 林业科学研究, 1995, 8 (专刊) : 13～18
[7]	周朝鸿,王自力,王绍云,等. 紫胶虫核型的初步研究[A]. 资源昆虫研究进展[C]. 云南:云南科技出版社, 1999: 36～42
[8]	李学斌. RAPD技术在动物遗传变异中的应用[J]. 云南畜牧兽医, 1997 (2) : 34～37
[9]	龚鹏,杨效文,谭声江,等. 分子遗传标记技术及其在昆虫科学中的应用[J]. 昆虫知识, 2001, 38 (2) : 86～91
[10]	蒋国芳,陆敢,黄琨,等. 用RAPD标记研究蚱属5 个种间的亲缘关系[J]. 昆虫学报, 2002, 45 (4) : 499～502
[11]	郑哲民,黄刚. 螽蟖总科部份属种RAPD带型变异的比较研究[J]. 陕西师范大学学报(自然科学版) , 2000, 28 (3) : 96～99
[12]	Black IV W C, DuTeau N M, Puterka G J, et al. Use of the random amp lified polymorphic DNA polymerase chain reaction(RAPD-PCR) to detectDNA polymorphisms in aphids[J]. BulletinEntomological Research, 1992, 82: 151～159
[13]	朱道弘,安藤喜一,城田安幸. 利用RAPD对稻蝗属昆虫亲缘关系的研究[J]. 昆虫学报, 2001, 44 (3) : 316～320
[14]	田英芳,黄刚,郑哲民,等. 一种简易的昆虫基因组DNA提取方法[J]. 陕西师范大学学报(自然科学版) , 1999, 27 (4) : 82～84
[15]	杨子祥,冯颖,陈晓鸣. 一种有效的蚜虫基因组DNA提取方法[J]. 林业科学研究, 2005, 18 (5) : 641～643
[16]	洪广基. 田紫胶虫(Kerria ruralisWang)黄色型分离培育初探[J]. 资源昆虫, 1986 (1) : 44～46
[17]	章士美. 昆虫的分布区系[J]. 江西农业大学学报, 1984, 18(01) : 11～17
[18]	许再富. 古今演变中的云南植物若干特点探讨[J]. 广西植物,2003, 23 (4) : 299～302
[19]	王子清. 中国动物志,昆虫纲,第二十二卷, 同翅目, 蚧总科[M]. 北京:科学出版社, 2001
[20]	田英芳,郑哲民. 七种蟋蟀基因组DNA 的RAPD 多态性研究[J]. 昆虫分类学报, 2001, 23 (4) : 248～252
[21]	Zhang Sufang, Yang Xiaowen, Ma J isheng. A study on genetic distance among different taxa of aphids (Homop tera: Aphidoidea)[J]. Entomologia Sinica, 2000, 7 (3) : 235～242
[22]	陈永久,张亚平. 随机扩增多态DNA影响因素的研究[J]. 动物学研究, 1997, 18 (2) : 211～227
[23]	成新跃,周红章,张广学. 分子生物学技术在昆虫系统学研究的应用[J]. 动物分类学报, 2000, 25 (2) : 121～133

Analysis of Relationships among the Main Commercial Species of Lac Insects Using Random Amplified Polymorphic DNA (RAPD)

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views