Comporison of Genetic Variations of Black Poplar (Populus nigra L.) Gene Resource by Microsatellite Markers

ZHANG Xiang-hua; SU Xiao-hua; HUANG Qin-jun; ZHANG Bing-yu

The simple sequence repeat(SSR) or microsatellite marker is currently preferred in genetic analysis due to its highly desirable properties.13 pairs of SSR primers were used to detect genetic diversity among 120 Populus nigra L.gene resource.A total of 171 allels were observed among the polymorphic loci,7～19 SSR alleles were detected for each locus with an average of 13.2 alleles per locus.13 pairs of SSR primers were polymorphic with the polymorphism information content(PIC) ranging from 0.396 to 0.909.The 120 clones were clustered based on the banding patterns generated by 13 polymorphic SSR loci.In general,this study indicates that SSRs are sufficiently abundant and polymorphic to be useful genetic markers in genetic diversity study of Populus nigra L.gene resources.This study provided theoretic basis for efficient utilization of these gene resources.

1. Research Institute of Forestry, CAF, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China

Received Date: 2005-01-06

Accepted Date: 2006-01-10

Abstract: The simple sequence repeat(SSR) or microsatellite marker is currently preferred in genetic analysis due to its highly desirable properties.13 pairs of SSR primers were used to detect genetic diversity among 120 Populus nigra L.gene resource.A total of 171 allels were observed among the polymorphic loci,7～19 SSR alleles were detected for each locus with an average of 13.2 alleles per locus.13 pairs of SSR primers were polymorphic with the polymorphism information content(PIC) ranging from 0.396 to 0.909.The 120 clones were clustered based on the banding patterns generated by 13 polymorphic SSR loci.In general,this study indicates that SSRs are sufficiently abundant and polymorphic to be useful genetic markers in genetic diversity study of Populus nigra L.gene resources.This study provided theoretic basis for efficient utilization of these gene resources.

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Comporison of Genetic Variations of Black Poplar (Populus nigra L.) Gene Resource by Microsatellite Markers

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