Effect of High Level of 6-BA on the Endogenous Hormones Level Changes in the Vitrification Seedling of Prunus cerasus

GAO Hong-bing; TANG Xiao-jie; MENG Qing-fan

This paper studied the effects of different levels of 6-BA on the vitrification rate of Prunus cerasus and the changes of endogenous hormones level,determined endogenous hormones content in the buds and callus by EnZRyme-linked Immunosorbent Assays.The results showed that the high level of 6-BA would raise the vitrification rate;as 6-BA content was 0.5 mg·L-1,the vitrification rate was 0,as 6-BA content added to 3.0 mg·L-1,the vitrification rate was 95%.In callus of the vitrification seedling,IAA and GA3level were higher than that of the normal seedling,ZR content was the same;In buds of the vitrification seedling,ZR level was lower than that of the normal seedling,IAA and GA3 contents were equal.The high level of 6-BA would cause the dislocation of the endogenous hormones rate in the vitrification seedling.

1. Forestry College of Beihua University, Jilin City 132013, Jilin, China

Received Date: 2005-08-10

Abstract: This paper studied the effects of different levels of 6-BA on the vitrification rate of Prunus cerasus and the changes of endogenous hormones level,determined endogenous hormones content in the buds and callus by EnZRyme-linked Immunosorbent Assays.The results showed that the high level of 6-BA would raise the vitrification rate;as 6-BA content was 0.5 mg·L-1,the vitrification rate was 0,as 6-BA content added to 3.0 mg·L-1,the vitrification rate was 95%.In callus of the vitrification seedling,IAA and GA3level were higher than that of the normal seedling,ZR content was the same;In buds of the vitrification seedling,ZR level was lower than that of the normal seedling,IAA and GA3 contents were equal.The high level of 6-BA would cause the dislocation of the endogenous hormones rate in the vitrification seedling.

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Reference (14)

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Effect of High Level of 6-BA on the Endogenous Hormones Level Changes in the Vitrification Seedling of Prunus cerasus

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