Key Technology Study on Oncidium Industrial Propagation by Tissue Culture

The key technology of Oncidium's industrial propagation were studied by in situ investigating the effects of the key factors such as minimal medium(improved MS,improved KC,V&W and self-prepared ZW series,etc.),phytohormone(6-BA,NAA,IBA,KT),additive(vitamin C,peptone,mashed banana,active carbon,sugar,etc.),on the mass breeding in every stage during Oncidium's industrial propagation.The results revealed that:(1)The protocorm like body(PLB) could be induce and increase as long as the concentration of 6-BA between 0.1～2 mg·L-1 and NAA 0.1～1.0 mg·L-1.At the revulsive initial stage a concentration of 6-BA 2 mg·L-1 and with NAA 0.2 mg·L-1 was helpful to increase the propagation basic number of protocorm like body.The multiply and differentiation of PLB could take place as long as 6-BA 0.05～0.1 mg·L-1 or addition of NAA 0.1 mg·L-1.The root emerging could be accelerated and the roots become stronger cooperation of addition of low concentration 6-BA(0.0～10.1 mg·L-1) together with NAA or IBA(0.1 mg·L-1).(2)The low concentration of auxin(0.01～0.1 mg·L-1) and the sort of low concentration of mineral medium(such as V&W,KC,etc.) seemed to benefit the decrease of the malformation during PLB differentiation and seedling growth.(3)In order to reduce the explant browning addition of active carbon 1 000 mg·L-1 and vitamin C 30 mg·L-1 was necessary.Adding peptone 5001 000 mg·L-1 or drawn substance of yeast seemed to promote the induction of PLB.Mashed banana 100 g·L-1 might benefit the PLB differentiation and improve the quality of test-tube plantlet.(4)Minimal mediums had notable effects on every stage of Oncidium tissue culture.The self-prepared ZW mediums with middle concentration of salt were more suitable to Oncidium propagation by tissue culture.(5)Oncidium was autotrophic without sugar in the medium.