Isolation of Endophytes and Screen of Antagonistic Strains in Poplar Trees

YUAN Xiu-ying; BAI Hong-xia; BAI Yu-ming; HU-Dagula; LIU Jun-xia

In a survey of endophytic fungi associated with Populus spp.in Huhhot,Inner Mengolia,154 fungal strains were isolated from leaves(bud),teguments and branches.The strains were classified into 13 different genera based on their morphological characteristics.The authous tested the antagonistic action of the 154 endophytic fungi on the pathogen,Cytospora sp.with the dual culture,germination of spores and inoculation experiments.The results showed that all endophytic fungi tested can inhibite,in some content,mycelium growth and spore germination of Cytospora sp.respectively.The strains of Penicillium sp.had the strongest antifungal effictiveness on the pathogen.In the antagonistic test,the colonial growth of Cytospora sp.was compressed and covered by the fast mycelial growth of Penicillium sp.So the development of Cytospora sp.was inhibited.In spore germination experiment,the filtrate from Penicillium sp.cultivated in liquid for three days also inhibited the growth of Cytospora sp.Therefore,the strain of Penicillium sp.could have a potential capability for biological control.

1. Forestry College of Inner Mongolia Agricultural University, Huhehot 010019, Inner Mongolia, China

Received Date: 2006-08-04

Abstract: In a survey of endophytic fungi associated with Populus spp.in Huhhot,Inner Mengolia,154 fungal strains were isolated from leaves(bud),teguments and branches.The strains were classified into 13 different genera based on their morphological characteristics.The authous tested the antagonistic action of the 154 endophytic fungi on the pathogen,Cytospora sp.with the dual culture,germination of spores and inoculation experiments.The results showed that all endophytic fungi tested can inhibite,in some content,mycelium growth and spore germination of Cytospora sp.respectively.The strains of Penicillium sp.had the strongest antifungal effictiveness on the pathogen.In the antagonistic test,the colonial growth of Cytospora sp.was compressed and covered by the fast mycelial growth of Penicillium sp.So the development of Cytospora sp.was inhibited.In spore germination experiment,the filtrate from Penicillium sp.cultivated in liquid for three days also inhibited the growth of Cytospora sp.Therefore,the strain of Penicillium sp.could have a potential capability for biological control.

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Reference (15)

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Isolation of Endophytes and Screen of Antagonistic Strains in Poplar Trees

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