Study on Volatile OilComponents and Activities of Restrainingand Eliminating Supper Oxygen Anion

HE Kai-yue; LI Xiao-chu; ZHANG Shuang-quan; FAN Ying-xin; QIU Jin- fen; BI Hiu-min

The vo latile oil from leaves o fT soong iodendron odorum w as iso lated by organic so lvent ex traction and itscomponen ts w ere ana lyzed and quantified by GC /MS /Computer. Meanwh ile, its activ ities of restra ining and elim ina??t ing ultra oxygen an ion w ere detected. The 38 compounds in T . odorum were identified and the m ain constituentsw ere ester ( 33. 396% ), terpeno ids ( 18. 313% ) , a lcohol ( 12. 065% ) and aromatic compounds ( 9. 484% ), Theresu lts show ed that therew ere strong functions of restraining and eliminating ultra oxygen an ion. When the vo latileo ilw as d iluted by 100 times, the act iv ity o f restraining ultra oxygen an ion reached max imum, and its activ ity w asstronger than that o f PG and PA . When d iluted 20 times, the act iv ity of to tal-SOD atta ined 495. 1U mL, amongw hich the CuZn-SOD w as 338. 50U mL-1, the 68. 36% of T-SOD; Mn-SOD w as 156. 70 U mL-1, the 31??64% ofT-SOD.

1. Nanjing Forestry University, Nanjing?? 210037, Jiangsu, China

2. Jiangsu Academy of Forestry, Nanjing?? 211153, Jiangsu, China

3. Nanjing NormalU n ivers ity, Nanjing?? 210097, Jiangsu, China

Received Date: 2006-01-25

Accepted Date: 2006-09-20

Abstract: The vo latile oil from leaves o fT soong iodendron odorum w as iso lated by organic so lvent ex traction and itscomponen ts w ere ana lyzed and quantified by GC /MS /Computer. Meanwh ile, its activ ities of restra ining and elim ina??t ing ultra oxygen an ion w ere detected. The 38 compounds in T . odorum were identified and the m ain constituentsw ere ester ( 33. 396% ), terpeno ids ( 18. 313% ) , a lcohol ( 12. 065% ) and aromatic compounds ( 9. 484% ), Theresu lts show ed that therew ere strong functions of restraining and eliminating ultra oxygen an ion. When the vo latileo ilw as d iluted by 100 times, the act iv ity o f restraining ultra oxygen an ion reached max imum, and its activ ity w asstronger than that o f PG and PA . When d iluted 20 times, the act iv ity of to tal-SOD atta ined 495. 1U mL, amongw hich the CuZn-SOD w as 338. 50U mL-1, the 68. 36% of T-SOD; Mn-SOD w as 156. 70 U mL-1, the 31??64% ofT-SOD.

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Reference (17)

[1]	守朝框, 徐荣章, 张清华. 中国珍稀濒危保护植物[M] . 北京: 中国林业出版社, 1989: 175~ 177
[2]	M ario Tellez. Ext racts ofF lou rensia cernua ( L) : Volat ile con stitu entsand an tifunga,l an tialga,l and an titerm ite b ioactiv it ies[J] . Journ al ofC hem ical E cology, 2001, 27( 11 ): 2 263~ 2 273
[3]	Kum ud in iM, Kuhajek Jeann e M, S tu rtz George D, et a l. Vu lga roneB, the an tifungal con stituent in the steam??d ist illed fract ion ofArtem i??sia d oug lasiana [J]. Journal of Chem ical E cology, 2003, 29 ( 8 ) :1771 ~ 1780
[4]	孔垂华, 黄寿山, 胡飞. 胜红蓟化感作用研究V. 挥发油对真菌、昆虫和植物的生物活性及其化学成份[J] . 生态学报, 2001, 21( 4 ): 584~ 587
[5]	江琰, 刘克武, 刘晓雯. 几种植物芳香油对食用油脂抗氧化作用的研究[J] . 粮油加工, 2000, 66( 2 ) : 14~ 15
[6]	潘家祜, 江明华. 生化药理学[M] . 上海: 复旦大学出版社,2004: 76~ 114
[7]	刘约权, 李贵深. 实验化学(上册) [M]. 北京: 高等教育出版社,1999: 48~ 84
[8]	郝小燕, 洪鑫, 余珍, 等. 观光木和云南含笑精油化学成分的研究和比较[J] . 贵州科学, 1999, 17 ( 4) : 287 ~ 290
[9]	宋晓凯, 吴立军, 屠鹏飞. 近五年木兰科植物生物活性研究及应用进展[J] . 中草药, 2002, 33 ( 10) : 958 ~ 960
[10]	张根旺, 毕艳兰, 徐学兵. 植酸及其它几种抗氧化剂抗氧化作用的研究[J] . 中国粮油学报, 1996, 11( 4 ): 45~ 49
[11]	李中, 杨建会, 史霖. 没食子酸丙酯对三硝基甲苯所致肝损伤的保护作用[J]. 卫生毒理学杂志, 1998, 12( 3 ) : 155~ 156
[12]	赵新淮, 刘宏芳, 王海亮. 几种植物抗氧化作用的化学生物学评价[J] . 东北农业大学学报, 2003, 34 ( 4) : 368 ~ 371
[13]	冯仁田, 何维, 越智宏论. 几种药食兼用植物提取物抗氧化作用的体外研究[J] . 中药材, 2000, 23( 11) : 690 ~ 693
[14]	曾小玲. 七种菊科植物抗活性氧作用的研究对超氧阴离子自由基的清除作用[J] . 中国现代医学杂志, 1999, 9 ( 2) : 44~ 52
[15]	蒋惠娣, 季燕萍, 张水利. 紫珠属药用植物体外抗氧化作用[J]. 中药材, 1999, 22( 3 ) : 139~ 141
[16]	林文洁, 陈丽晖. 植物体中的自由基[J] . 海南大学学报自然科学版, 1998, 16( 4 ): 370~ 375
[17]	黎瑞珍, 杨庆建, 陈贻锐. 抗超氧化物歧化酶( SOD)活性测定及其应用研究[J] . 琼州大学学报, 2004, 11( 5 ) : 34~ 36

Study on Volatile OilComponents and Activities of Restrainingand Eliminating Supper Oxygen Anion

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