Effect of Mechanical Wounding on the Content of HydrogenPeroxide in Ash leaf Maple Seedlings

ZHANG Wen; SHEN Ying-bai; SHEN Yuan-yuan

Using ashleafmaple (Acer negundo ) seed lings as materials, DAB tissue coloration and spectropho tometryasm ethods, the autho rs exam ined and compared the dynam ic concentrations o fH2O2 in d ifferent leaves: the dam??aged leaves, the intact leaves of the ident ical damaged plant and signa l induced leaves o f the neighboring plantsdamaged by 0, 15, 30, 60, 120, 240m inutes. The resu lts show ed that themechanicalwound ing cou ld induce the sys??tem ic concentrations of H2O2, the contents susta ining increased in 60m inutes, and reached the m ax po int at 240m inutes. A fter using theM eJA ( 1 ??mo l·L-1 ) to treat the hea lthy p lants, the concen trat ions o fH2O2 increased dis??t inctly. It could be concluded thatMeJA induced the sim ilar defensive system s instead o fmechan ica lw ounding, byw ay of initial signa ls in plants, and reached the effects of defending env ironmen tw ound.

1. College of Biolog ical Scien ce and Biotechnology, Beijing Forestry U n ivers ity , Beijing ?? 100083

Received Date: 2006-03-10

Abstract: Using ashleafmaple (Acer negundo ) seed lings as materials, DAB tissue coloration and spectropho tometryasm ethods, the autho rs exam ined and compared the dynam ic concentrations o fH2O2 in d ifferent leaves: the dam??aged leaves, the intact leaves of the ident ical damaged plant and signa l induced leaves o f the neighboring plantsdamaged by 0, 15, 30, 60, 120, 240m inutes. The resu lts show ed that themechanicalwound ing cou ld induce the sys??tem ic concentrations of H2O2, the contents susta ining increased in 60m inutes, and reached the m ax po int at 240m inutes. A fter using theM eJA ( 1 ??mo l·L-1 ) to treat the hea lthy p lants, the concen trat ions o fH2O2 increased dis??t inctly. It could be concluded thatMeJA induced the sim ilar defensive system s instead o fmechan ica lw ounding, byw ay of initial signa ls in plants, and reached the effects of defending env ironmen tw ound.

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Reference (14)

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Effect of Mechanical Wounding on the Content of HydrogenPeroxide in Ash leaf Maple Seedlings

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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