Applica tion of EST-CAPSMarkers to GeneticMapping inEuca lyptus u rophylla and Euca lyptus te re ticorn is

ZHANG Zhao-yuan; GAN Si-ming; LI Fa-gen; LI Mei; WENG Qi-jie; HU Zhe-sen

Volume 20 Issue 2

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Applica tion of EST-CAPSMarkers to GeneticMapping inEuca lyptus u rophylla and Euca lyptus te re ticorn is

1.
Research Institute of Trop ical Forestry, CAF, Guangzhou　510520, Guangdong, China
2.
Forestry Faculty, Fujian University of Agriculture and Forestry, Fuzhou　350002, Fujian, China

Abstract

Seven EST-CAPS markerswere identified from 54 candidate ESTs for genetic mapp ingwith an F1 population of Eucalyptus urophylla ×E. tereticornis, including three specific in female parent (one being aberrant from normal segregation among the sibs) , two specific in male parent, and two shared by both parents. Four EST2CAPSmarkers were integrated to independent linkage group s on the RAPD linkage map of E. urophylla constructed p reviously, which contributed to some degrees to the extension of linkage group length. Also, four EST2CAPSs weremapped to different linkage group s on the RAPD linkage map of E. tereticornis, which gave rise to the increase oflinkage group length. In addition, the aberrantmarkerwas found to be unlinked to any linkage group in E. urophylla. This study demonstrated the usefulness of EST-CAPSmarkers in genetic mapp ing in Euca lyptus.
- EST-CAPS,
- Eucalyptus urophylla,
- Eucalyptus tereticorn is,
- genetic map

References

[1]	白嘉雨. 中国热带地区桉属树种的遗传改良回顾[A]. 见:洪菊生,王豁然. 澳大利亚阔叶树研究[M]. 北京:中国林业出版社,1993: 33～48
[Z]. Whitehead Institute, CambridgeMA: Research Technical, 1992
[3]	吴坤明,吴菊英,甘四明,等. 桉树种间杂种的比较和选择研究[J]. 林业科学研究, 2002, 15 (1) : 1～6
[4]	徐建民,白嘉雨,甘四明. 尾叶桉家系综合选择研究[J]. 林业科学研究, 1996, 9 (6) : 561～567
[5]	李光友,徐建民,陆钊华,等. 尾叶桉二代测定林家系的综合评选[J]. 林业科学研究, 2005, 18 (1) : 57～61
[6]	徐建民,陆钊华,李光友,等. 细叶桉种源—家系综合选择的研究[J]. 林业科学研究, 2003, 16 (1) : 1～7
[7]	Grattapaglia D, Sederoff R. Genetic linkage map s of Eucalyptus grandis and Eucalyptus urophylla using a p seudo-testcrossmapp ing strategy and RAPD markers [J]. Genetics, 1994, 137: 1127～1137
[8]	Verhaegen D, Plomion C. Genetic mapp ing in Eucalyptus urophyllaand Eucalyptus grandis using RAPD markers [J]. Genome, 1996,39: 1051～1061
[9]	Brondani R P V, Brondani C, Tarchini R, et al. Development, characterization and mapp ing of microsatellite markers in Eucalyptusgrandis and Eucalyptus urophylla [J]. Theor App l Genet, 1998, 97:816～827
[10]	Byrne M,Murrell J C, Allen B, et al. An integrated genetic linkagemap for Eucalyptus using RFLP, RAPD and isozyme markers [J].TheorApp l Genet, 1995, 91: 869～875
[11]	Margues CM,Araujo J A, Ferreira J G, et al. AFLP genetic map s ofEucalyptus globulus and E. tereticornis[J]. Theor App l Genet, 1998,96: 727～737
[12]	Bundock P C, HaydenM,Vaillancourt R E. Linkage map s of Eucalyptus globulus using RAPD and microsatellite markers [J]. SilvaeGenetica, 2000, 49: 223～232
[13]	Thamarus KA, Groom K,Murrell J, et al. A genetic linkage map forEucalyptus globulus with candidate loci for wood, fibre, and floraltraits [J]. TheorApp l Genet, 2002, 104: 379～387
[14]	Gan S, Shi J,LiM, et al. Moderate-densitymolecularmap s of Eucalyptus urophylla S. T. Blake and E. tereticornis Smith genomes basedon RAPD markers [J]. Genetica, 2003, 118: 59～67
[15]	Agrama H A, George T L, Salah S F. Construction of genome mapfor Eucalyptus cam aldulensis Dehn [J]. Silvae Genetica, 2002, 51:201～206
[16]	Tragoonrung S, Kanazin V, Hayes PM, et al. Sequence-tagged-sitefacilitated PCR for barley genome mapp ing [J]. Theor App l Genet,1992, 84: 1002～1008
[17]	Konieczny A, Ausubel F M. A p rocedure for mapp ing A rabidopsismutations using codominant ecotype-specific PCR-based markers[J]. Plant J, 1993, 4: 403～410
[18]	Nikaido AM,Ujino T, Iwata H, et al. AFLP and CAPS linkagemap sof Cryptom eria japonica [J]. Theor App l Genet, 2000, 100: 825～831
[19]	Tani N, Takahashi T, Iwata H, et al. A consensus linkage map forsugi (Cryptom eria japonica) from two pedigrees based on microsatellites and exp ressed sequence tags [J]. Genetics, 2003, 165: 1551～1568
[20]	Temesgen B,Brown G R, Harry D E, et al. Genetic mapp ing of exp ressed sequence tag polymorphism ( ESTP) markers in loblolly p ine( Pinus taeda L. ) [J]. TheorApp l Genet, 2001, 102: 664～675
[21]	AcheréV, Rampant P F, Jeandroz S, et al. A full saturated linkagemap of Picea abies including AFLP, SSR, ESTP, 5S rDNA and morphologicalmarkers [J]. TheorApp l Genet, 2004, 108: 1602～1613
[22]	Doyle J J,Doyle J L. Isolation of p lant DNA from fresh tissue [J].Focus, 1990, 12: 13～14
[23]	Lincoln S E, Daly M J, Lander E S. Constructing genetic linkagemap swithMAPMAKER /EXP 3. 0
[24]	Voorrip s R E. MapChart: software for the graphical p resentation oflinkage map s and QTLs [J]. J Hered, 2002, 93: 77～78
[25]	Strabala T J. Exp ressed sequence tag databases from forestry treespecies [A]. In: Kumar S, Fladung M. Molecular Genetics andBreeding of Forest Trees [C]. New York: Food Products Press,2004: 19～51
[26]	Carmargo L E. The FORESTs genome p roject in perspective [A].Eucalyp t GenomicMeeting, Hobart, Tasmania, Australia, 2004
[27]	HarryD E, Temesgen B,Neale D B. Codominant PCR2based markers for Pinus taeda developed from mapped cDNA clones[J]. TheorApp l Genet, 1998, 97: 327～336
[28]	Tsumura Y, Tomaru N. Genetic diversity of Cryptom eria japonica using co-dominantDNA markers based on sequenced tagged sites[J].TheorApp l Genet, 1999, 98: 396～404
[29]	Plomion C, Hurme P, Frigerio JM, et al. Develop ing SSCP markers in two Pinus species [J]. Mol Breed, 1999, 5: 21～31

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Applica tion of EST-CAPSMarkers to GeneticMapping inEuca lyptus u rophylla and Euca lyptus te re ticorn is

1. Research Institute of Trop ical Forestry, CAF, Guangzhou　510520, Guangdong, China

2. Forestry Faculty, Fujian University of Agriculture and Forestry, Fuzhou　350002, Fujian, China

Abstract: Seven EST-CAPS markerswere identified from 54 candidate ESTs for genetic mapp ingwith an F1 population of Eucalyptus urophylla ×E. tereticornis, including three specific in female parent (one being aberrant from normal segregation among the sibs) , two specific in male parent, and two shared by both parents. Four EST2CAPSmarkers were integrated to independent linkage group s on the RAPD linkage map of E. urophylla constructed p reviously, which contributed to some degrees to the extension of linkage group length. Also, four EST2CAPSs weremapped to different linkage group s on the RAPD linkage map of E. tereticornis, which gave rise to the increase oflinkage group length. In addition, the aberrantmarkerwas found to be unlinked to any linkage group in E. urophylla. This study demonstrated the usefulness of EST-CAPSmarkers in genetic mapp ing in Euca lyptus.

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Reference (29)

[1]	白嘉雨. 中国热带地区桉属树种的遗传改良回顾[A]. 见:洪菊生,王豁然. 澳大利亚阔叶树研究[M]. 北京:中国林业出版社,1993: 33～48
[2]	[Z]. Whitehead Institute, CambridgeMA: Research Technical, 1992
[3]	吴坤明,吴菊英,甘四明,等. 桉树种间杂种的比较和选择研究[J]. 林业科学研究, 2002, 15 (1) : 1～6
[4]	徐建民,白嘉雨,甘四明. 尾叶桉家系综合选择研究[J]. 林业科学研究, 1996, 9 (6) : 561～567
[5]	李光友,徐建民,陆钊华,等. 尾叶桉二代测定林家系的综合评选[J]. 林业科学研究, 2005, 18 (1) : 57～61
[6]	徐建民,陆钊华,李光友,等. 细叶桉种源—家系综合选择的研究[J]. 林业科学研究, 2003, 16 (1) : 1～7
[7]	Grattapaglia D, Sederoff R. Genetic linkage map s of Eucalyptus grandis and Eucalyptus urophylla using a p seudo-testcrossmapp ing strategy and RAPD markers [J]. Genetics, 1994, 137: 1127～1137
[8]	Verhaegen D, Plomion C. Genetic mapp ing in Eucalyptus urophyllaand Eucalyptus grandis using RAPD markers [J]. Genome, 1996,39: 1051～1061
[9]	Brondani R P V, Brondani C, Tarchini R, et al. Development, characterization and mapp ing of microsatellite markers in Eucalyptusgrandis and Eucalyptus urophylla [J]. Theor App l Genet, 1998, 97:816～827
[10]	Byrne M,Murrell J C, Allen B, et al. An integrated genetic linkagemap for Eucalyptus using RFLP, RAPD and isozyme markers [J].TheorApp l Genet, 1995, 91: 869～875
[11]	Margues CM,Araujo J A, Ferreira J G, et al. AFLP genetic map s ofEucalyptus globulus and E. tereticornis[J]. Theor App l Genet, 1998,96: 727～737
[12]	Bundock P C, HaydenM,Vaillancourt R E. Linkage map s of Eucalyptus globulus using RAPD and microsatellite markers [J]. SilvaeGenetica, 2000, 49: 223～232
[13]	Thamarus KA, Groom K,Murrell J, et al. A genetic linkage map forEucalyptus globulus with candidate loci for wood, fibre, and floraltraits [J]. TheorApp l Genet, 2002, 104: 379～387
[14]	Gan S, Shi J,LiM, et al. Moderate-densitymolecularmap s of Eucalyptus urophylla S. T. Blake and E. tereticornis Smith genomes basedon RAPD markers [J]. Genetica, 2003, 118: 59～67
[15]	Agrama H A, George T L, Salah S F. Construction of genome mapfor Eucalyptus cam aldulensis Dehn [J]. Silvae Genetica, 2002, 51:201～206
[16]	Tragoonrung S, Kanazin V, Hayes PM, et al. Sequence-tagged-sitefacilitated PCR for barley genome mapp ing [J]. Theor App l Genet,1992, 84: 1002～1008
[17]	Konieczny A, Ausubel F M. A p rocedure for mapp ing A rabidopsismutations using codominant ecotype-specific PCR-based markers[J]. Plant J, 1993, 4: 403～410
[18]	Nikaido AM,Ujino T, Iwata H, et al. AFLP and CAPS linkagemap sof Cryptom eria japonica [J]. Theor App l Genet, 2000, 100: 825～831
[19]	Tani N, Takahashi T, Iwata H, et al. A consensus linkage map forsugi (Cryptom eria japonica) from two pedigrees based on microsatellites and exp ressed sequence tags [J]. Genetics, 2003, 165: 1551～1568
[20]	Temesgen B,Brown G R, Harry D E, et al. Genetic mapp ing of exp ressed sequence tag polymorphism ( ESTP) markers in loblolly p ine( Pinus taeda L. ) [J]. TheorApp l Genet, 2001, 102: 664～675
[21]	AcheréV, Rampant P F, Jeandroz S, et al. A full saturated linkagemap of Picea abies including AFLP, SSR, ESTP, 5S rDNA and morphologicalmarkers [J]. TheorApp l Genet, 2004, 108: 1602～1613
[22]	Doyle J J,Doyle J L. Isolation of p lant DNA from fresh tissue [J].Focus, 1990, 12: 13～14
[23]	Lincoln S E, Daly M J, Lander E S. Constructing genetic linkagemap swithMAPMAKER /EXP 3. 0
[24]	Voorrip s R E. MapChart: software for the graphical p resentation oflinkage map s and QTLs [J]. J Hered, 2002, 93: 77～78
[25]	Strabala T J. Exp ressed sequence tag databases from forestry treespecies [A]. In: Kumar S, Fladung M. Molecular Genetics andBreeding of Forest Trees [C]. New York: Food Products Press,2004: 19～51
[26]	Carmargo L E. The FORESTs genome p roject in perspective [A].Eucalyp t GenomicMeeting, Hobart, Tasmania, Australia, 2004
[27]	HarryD E, Temesgen B,Neale D B. Codominant PCR2based markers for Pinus taeda developed from mapped cDNA clones[J]. TheorApp l Genet, 1998, 97: 327～336
[28]	Tsumura Y, Tomaru N. Genetic diversity of Cryptom eria japonica using co-dominantDNA markers based on sequenced tagged sites[J].TheorApp l Genet, 1999, 98: 396～404
[29]	Plomion C, Hurme P, Frigerio JM, et al. Develop ing SSCP markers in two Pinus species [J]. Mol Breed, 1999, 5: 21～31

Applica tion of EST-CAPSMarkers to GeneticMapping inEuca lyptus u rophylla and Euca lyptus te re ticorn is

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通讯作者: 陈斌, bchen63@163.com

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Applica tion of EST-CAPSMarkers to GeneticMapping inEuca lyptus u rophylla and Euca lyptus te re ticorn is

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