Genetic Variation of Strains of Cryphonectriaparasitica from Korea, USA and China

PIAO Chun-gen; KIM Kyung-hee; LEE Sang-hyun; LEE Sang-hyun; MOON Byung-ju

Genetic variation of 26 stra ins of Cryphonectria parasitica from Korea, USA and Ch ina w as investigatedusing random amplified po lymorph ic DNA (RAPD) of genom ic DNA. 115 RAPD bandsw ere amplified by po lymer??ase cha in react ion w ith 12 random primers, and 61. 7% o f wh ich w ere polymorphic, the size of amp lified RAPDbands ranged from 0. 19 to 3. 1 kb. The lineage of 26 strains based on cluster analysis of RAPD bands, could beclassified as 1 b ig g roup and the big group inc luded 17 stra ins o fKo rea and 4 strains ofUSA. From th is resu l,t itw as show ed thatmost stra ins ofKoreaw ere sim ilar to each other, and also sim ilar to strains o fUSA, and the geneticre lationsh ip w ith stra in o f China w as very low.

1. R esearch In stitute of Forest Ecology, Environmen t and Protection, CAF, K ey Laboratory of Forest Protection of State ForestryAdministrat ion, Bei jing?? 100091, China

2. Department of Forest Diseases and Pests, Korea Forest Research Institute, Seoul?? 130-712, Korea

3. Faculty of Natural Resou rces and Life Science, Dong??A University, Pusan?? 604-714, Korea

Abstract: Genetic variation of 26 stra ins of Cryphonectria parasitica from Korea, USA and Ch ina w as investigatedusing random amplified po lymorph ic DNA (RAPD) of genom ic DNA. 115 RAPD bandsw ere amplified by po lymer??ase cha in react ion w ith 12 random primers, and 61. 7% o f wh ich w ere polymorphic, the size of amp lified RAPDbands ranged from 0. 19 to 3. 1 kb. The lineage of 26 strains based on cluster analysis of RAPD bands, could beclassified as 1 b ig g roup and the big group inc luded 17 stra ins o fKo rea and 4 strains ofUSA. From th is resu l,t itw as show ed thatmost stra ins ofKoreaw ere sim ilar to each other, and also sim ilar to strains o fUSA, and the geneticre lationsh ip w ith stra in o f China w as very low.

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Reference (7)

[R]. 韩国林业研究院, 1999: 384~ 393
[2]	K im K H, Lee S H, Lee SM, et a l. 1999年度林业研究事业报告书(山林环境5-1 )
[3]	Anagnostakis S L. C rphonectria parasit ica carse of chestnu t b ligh t[J] . Advances in Plant Patho logy, 1988, 6: 123~ 136
[4]	H ein igerU, R igl ing D. B iological con trol of chestnu tb light in Eu rope[J] . Annu Rev Phytopatho,l 1994, 32: 581~ 599
[5]	W ron sk iR, Kudera U, W ih elm E. C haracterizat ion of Cryphon ectriapa rasitica strains by random am p lijied polym orph ic DNA ( RAPD )techn iqu e and convent ion alm ethods [J]. Eu r J For Path, 1997, 27( 2 ): 95 ~ 103
[6]	Lee S B, Tayler JW. Isolation of DNA from fungalmycel ia and s ingle spores[A] . in: A Gu id e to M ethods and App lication[M] . APSPress , S t. Pau lMN USA , 1990: 282~ 287
[7]	M ilgroom M G, W ang K, ZhouY, et al. Intercont inen tal popu lationstru cture of the ch estnut bl igh t fungus. Cryph onectria pa rasitica [J] .M ycolog ia, 1996, 88 ( 2) : 179~ 190

[1]	[R]. 韩国林业研究院, 1999: 384~ 393
[2]	K im K H, Lee S H, Lee SM, et a l. 1999年度林业研究事业报告书(山林环境5-1 )
[3]	Anagnostakis S L. C rphonectria parasit ica carse of chestnu t b ligh t[J] . Advances in Plant Patho logy, 1988, 6: 123~ 136
[4]	H ein igerU, R igl ing D. B iological con trol of chestnu tb light in Eu rope[J] . Annu Rev Phytopatho,l 1994, 32: 581~ 599
[5]	W ron sk iR, Kudera U, W ih elm E. C haracterizat ion of Cryphon ectriapa rasitica strains by random am p lijied polym orph ic DNA ( RAPD )techn iqu e and convent ion alm ethods [J]. Eu r J For Path, 1997, 27( 2 ): 95 ~ 103
[6]	Lee S B, Tayler JW. Isolation of DNA from fungalmycel ia and s ingle spores[A] . in: A Gu id e to M ethods and App lication[M] . APSPress , S t. Pau lMN USA , 1990: 282~ 287
[7]	M ilgroom M G, W ang K, ZhouY, et al. Intercont inen tal popu lationstru cture of the ch estnut bl igh t fungus. Cryph onectria pa rasitica [J] .M ycolog ia, 1996, 88 ( 2) : 179~ 190

Genetic Variation of Strains of Cryphonectriaparasitica from Korea, USA and China

Abstract

References

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