Establishment of Tissue-Culture Regeneration System in the Hybrid Poplar 110

CHEN Cai-xia; SHEN Xin; WANG Yi-ning; CHEN Shao-liang

Volume 20 Issue 2

Article Contents

Turn off MathJax

Article Navigation > Forest Research > 2007 > 20(2): 287-291

Citation:

Establishment of Tissue-Culture Regeneration System in the Hybrid Poplar 110

1.
School of Biological Science and Biotechnology, Beijing Forestry University,Beijing　100083, China

Abstract

The new emerging stems and buds of pop lar 110 ( Populus deltoids ×P. m ax im ow iczii cv‘Eridano’)were used as exp lants and incubated onMSmedia supp lemented with various hormone combinations. Once the successive and rooting p lants were obtained through selection of culturalmedium formula, leaves and internodals wereexcised from tubed-culture p lanlets and induced to form regenerated p lants. The result showedMS + 62BA 0. 5 mg·L-1 +NAA 0. 1 mg·L-1 + TDZ 0. 02 mg·L-1 was beneficial for leaves to inducing adventitious buds; The propermedium for using internodals as exp lantswasMS + 6-BA 0. 3 mg·L-1 +NAA 0. 05 mg·L-1 + TDZ 0. 02 mg·L-1 ;while the medium ofMS + 62BA 0. 3 mg·L-1 +NAA 0. 1 mg·L-1 was p roper to culturing the stronger tubedp lanlets. The op timum medium for p roducing rootswas 1 /2MS + IBA 0. 5 mg·L-1. The effect of different incisingmode and growth state of leaves and internodals on inducing adventitiouswas discussed. In vitro vegetative p ropagation and p lant rootingwere op timized to establish the high2efficient tissue culture rep roduction system of pop lar 110,which make it possible for tree breeding and genetic transformation of this species.
- hybrid pop lar 110 ( Popu lus deltoids × P. m ax im ow iczii cv ‘ Eridano ’)

References

[1]	林善枝,肖基浒,张志毅. 杨树分子标记研究进展[J]. 北京林业大学学报, 2000, 22 (6) : 79～83
[2]	赵华燕,卢善发,晁瑞堂. 杨树的组织培养及其基因工程研究[J]. 植物学通报, 2001, 18 (2) : 169～176
[3]	张绮纹,李金花. 杨树工业用材林新品种[M]. 北京:中国林业出版社, 2003
[4]	李世承,李晶,佟凤琴,等. 山杨、大青杨、毛白杨组织培养的研究[J]. 辽宁大学学报(自然科学报) , 1995, 22 (2) : 59～61
[5]	杜宁霞,李云,于海武,等. 毛白杨叶片再生系统的建立(英文)[J]. Forestry Studies in China, 2002, 2 (4) : 48～51
[6]	孙宇飞,高秀华,赵彦修,等. 欧美107杨组织培养再生系统的建立[J]. 山东师范大学学报(自然科学版) , 2004, 19 (2) : 85～87
[7]	马宗新,高玉祥,赵红,等. 三倍体速生杨树的组织培养及应用[J]. 安徽农业科学, 2004, 32 (4) : 715～716
[8]	于志水,金红,苘胜军,等. 黑杨派杨树组培再生系统的研究[J].辽宁林业科技, 2002 (6) : 11～13
[9]	张绮纹,苏晓华. 杨树定向遗传改良及高新技术育种[M]. 北京:中国林业出版社, 1999
[10]	Massimo Delledome, Gianni Allegro, Beatrice Belenghi, et al.Transformation of white pop lar( populus alba L. ) with a novel arabidop sis thaliana cysteine p roteinase inhibitor and analysis of insectpest resistance[J]. Molecular Breeding, 2001, 7: 35～42
[11]	In-Suk Ahn, Young-Goo Park, Dong-III Shin, et al. Genetic transformation of Populus nigra ×m axim ow iczii using agrobacterium tumefaciens harboring Antisense OMT gene [J]. Plant Biotechnology, 2001, 3 (3) : 135～140
[12]	陈维伦,郭东红,杨善英,等. 山新杨叶外植体的器官分化以及生长调节物质对它的影响[J]. 植物学报, 1980, 22 ( 4 ) : 311～315
[13]	NoelN, Lep le J C, Pilate G. Op timization of in vitromicrop ropagation and regeneration for Populus ×interam ericana and Populus ×euram ericana hybrida ( P. deltoides, P. trichocarpa, and P. nigra)[J]. Plant Cell Rep, 2002, 20: 1 150～1 155
[14]	Huetteman C A, Preece J E. Thidazuron: a poen cytokinin forwoody p lant tissue culture[J]. Plant Cell Tissue Org Cult, 1993,33: 105～119
[15]	诸葛强,王婕琛,黄敏仁,等. 新疆杨植株再生体系的建立[J].南京林业大学学报(自然科学版) , 2003, 27 (6) : 1～4
[16]	DaiW H, Cheng ZM, SargentW. Plant regeneration and agrobacterium-mediated transformation of two elite aspen hybrid clonesfrom in vitro leaf tissues[J]. In vitro Cell Dev Biol Plant, 2003,39: 6～11
[17]	In-Suk Ahn, Young-Goo Park, Dong-III Shin, et al. Identification ofexcision of Ac transposable element in P. nigra ×m axim ow iczii using agrobacterium-mediated transformation [J]. PlantBiotechnology, 2003, 5 (1) : 19～23
[18]	Lee2Stadelmann O Y, Lee Sw, HackettW P, et al. The formation ofadventitious buds in vitro on micro-cross sections of hybrid Populusleafmidveins[J]. Plant Sci , 1989, 61: 263～272
[19]	Cap ingM A, Steven H S, RichardMeilan. Agrobacterium-mediated transformation of the genome-sequenced pop lar clone, Nisqually-1 [J]. PlantMolecular Biology , 2004 , 22: 1～9
[20]	郑均宝,张玉满,杨文芝,等. 741杨离体叶片的再生及抗虫基因转化[J]. 河北农业大学学报, 1995, 118 (3) : 20～25
[21]	ConfalonieriM, BelenghiB, BalestrazziA, et al. Transformation ofelite white pop lar ( Populus alba L. ) cv. ‘Villafranca’and evaluation of herbicide resistance [J]. Plant Cell Reports , 2000, 19:978～982
[22]	Kyung-Hwan Han, Harvey D B, J r. Milton P G. Adventitious rootand shoot regeneration in vitro is undermajor gene controlin in a F-family of hybrid pop lar ( Populus trichocarpa ×P. deltoides) [J].Forest Genetics, 1994, 1 (3) : 139～146
[23]	Sha L, McCown B H, Peterson L A. Occurrence and cause ofShoot-tip necrosis in shoot culture [J]. J Am Soc Hort Sci, 1985,110: 631～634
[24]	Marc De Block. Factors influencing the tissue culture and theagrobacterium tumefaciens-mediated transformation of hybrid aspenand pop lar clones[J]. Plant Physiol, 1990, 93: 1 110～1 116

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(3150) PDF downloads(1359) Cited by()

Proportional views

Establishment of Tissue-Culture Regeneration System in the Hybrid Poplar 110

1. School of Biological Science and Biotechnology, Beijing Forestry University,Beijing　100083, China

Abstract: The new emerging stems and buds of pop lar 110 ( Populus deltoids ×P. m ax im ow iczii cv‘Eridano’)were used as exp lants and incubated onMSmedia supp lemented with various hormone combinations. Once the successive and rooting p lants were obtained through selection of culturalmedium formula, leaves and internodals wereexcised from tubed-culture p lanlets and induced to form regenerated p lants. The result showedMS + 62BA 0. 5 mg·L-1 +NAA 0. 1 mg·L-1 + TDZ 0. 02 mg·L-1 was beneficial for leaves to inducing adventitious buds; The propermedium for using internodals as exp lantswasMS + 6-BA 0. 3 mg·L-1 +NAA 0. 05 mg·L-1 + TDZ 0. 02 mg·L-1 ;while the medium ofMS + 62BA 0. 3 mg·L-1 +NAA 0. 1 mg·L-1 was p roper to culturing the stronger tubedp lanlets. The op timum medium for p roducing rootswas 1 /2MS + IBA 0. 5 mg·L-1. The effect of different incisingmode and growth state of leaves and internodals on inducing adventitiouswas discussed. In vitro vegetative p ropagation and p lant rootingwere op timized to establish the high2efficient tissue culture rep roduction system of pop lar 110,which make it possible for tree breeding and genetic transformation of this species.

HTML

Reference (24)

[1]	林善枝,肖基浒,张志毅. 杨树分子标记研究进展[J]. 北京林业大学学报, 2000, 22 (6) : 79～83
[2]	赵华燕,卢善发,晁瑞堂. 杨树的组织培养及其基因工程研究[J]. 植物学通报, 2001, 18 (2) : 169～176
[3]	张绮纹,李金花. 杨树工业用材林新品种[M]. 北京:中国林业出版社, 2003
[4]	李世承,李晶,佟凤琴,等. 山杨、大青杨、毛白杨组织培养的研究[J]. 辽宁大学学报(自然科学报) , 1995, 22 (2) : 59～61
[5]	杜宁霞,李云,于海武,等. 毛白杨叶片再生系统的建立(英文)[J]. Forestry Studies in China, 2002, 2 (4) : 48～51
[6]	孙宇飞,高秀华,赵彦修,等. 欧美107杨组织培养再生系统的建立[J]. 山东师范大学学报(自然科学版) , 2004, 19 (2) : 85～87
[7]	马宗新,高玉祥,赵红,等. 三倍体速生杨树的组织培养及应用[J]. 安徽农业科学, 2004, 32 (4) : 715～716
[8]	于志水,金红,苘胜军,等. 黑杨派杨树组培再生系统的研究[J].辽宁林业科技, 2002 (6) : 11～13
[9]	张绮纹,苏晓华. 杨树定向遗传改良及高新技术育种[M]. 北京:中国林业出版社, 1999
[10]	Massimo Delledome, Gianni Allegro, Beatrice Belenghi, et al.Transformation of white pop lar( populus alba L. ) with a novel arabidop sis thaliana cysteine p roteinase inhibitor and analysis of insectpest resistance[J]. Molecular Breeding, 2001, 7: 35～42
[11]	In-Suk Ahn, Young-Goo Park, Dong-III Shin, et al. Genetic transformation of Populus nigra ×m axim ow iczii using agrobacterium tumefaciens harboring Antisense OMT gene [J]. Plant Biotechnology, 2001, 3 (3) : 135～140
[12]	陈维伦,郭东红,杨善英,等. 山新杨叶外植体的器官分化以及生长调节物质对它的影响[J]. 植物学报, 1980, 22 ( 4 ) : 311～315
[13]	NoelN, Lep le J C, Pilate G. Op timization of in vitromicrop ropagation and regeneration for Populus ×interam ericana and Populus ×euram ericana hybrida ( P. deltoides, P. trichocarpa, and P. nigra)[J]. Plant Cell Rep, 2002, 20: 1 150～1 155
[14]	Huetteman C A, Preece J E. Thidazuron: a poen cytokinin forwoody p lant tissue culture[J]. Plant Cell Tissue Org Cult, 1993,33: 105～119
[15]	诸葛强,王婕琛,黄敏仁,等. 新疆杨植株再生体系的建立[J].南京林业大学学报(自然科学版) , 2003, 27 (6) : 1～4
[16]	DaiW H, Cheng ZM, SargentW. Plant regeneration and agrobacterium-mediated transformation of two elite aspen hybrid clonesfrom in vitro leaf tissues[J]. In vitro Cell Dev Biol Plant, 2003,39: 6～11
[17]	In-Suk Ahn, Young-Goo Park, Dong-III Shin, et al. Identification ofexcision of Ac transposable element in P. nigra ×m axim ow iczii using agrobacterium-mediated transformation [J]. PlantBiotechnology, 2003, 5 (1) : 19～23
[18]	Lee2Stadelmann O Y, Lee Sw, HackettW P, et al. The formation ofadventitious buds in vitro on micro-cross sections of hybrid Populusleafmidveins[J]. Plant Sci , 1989, 61: 263～272
[19]	Cap ingM A, Steven H S, RichardMeilan. Agrobacterium-mediated transformation of the genome-sequenced pop lar clone, Nisqually-1 [J]. PlantMolecular Biology , 2004 , 22: 1～9
[20]	郑均宝,张玉满,杨文芝,等. 741杨离体叶片的再生及抗虫基因转化[J]. 河北农业大学学报, 1995, 118 (3) : 20～25
[21]	ConfalonieriM, BelenghiB, BalestrazziA, et al. Transformation ofelite white pop lar ( Populus alba L. ) cv. ‘Villafranca’and evaluation of herbicide resistance [J]. Plant Cell Reports , 2000, 19:978～982
[22]	Kyung-Hwan Han, Harvey D B, J r. Milton P G. Adventitious rootand shoot regeneration in vitro is undermajor gene controlin in a F-family of hybrid pop lar ( Populus trichocarpa ×P. deltoides) [J].Forest Genetics, 1994, 1 (3) : 139～146
[23]	Sha L, McCown B H, Peterson L A. Occurrence and cause ofShoot-tip necrosis in shoot culture [J]. J Am Soc Hort Sci, 1985,110: 631～634
[24]	Marc De Block. Factors influencing the tissue culture and theagrobacterium tumefaciens-mediated transformation of hybrid aspenand pop lar clones[J]. Plant Physiol, 1990, 93: 1 110～1 116

Establishment of Tissue-Culture Regeneration System in the Hybrid Poplar 110

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

Establishment of Tissue-Culture Regeneration System in the Hybrid Poplar 110

HTML

Catalog

Export File

Citation

Format

Content