Construction of Full-length cDNA Library of MaleFloral Buds of Popu lus de ltoides

ZHOU Xiang-ming; ZHANG Bing-yu; SU Xiao-hua; WANG Da-hai; HUANG Qin-jun; ZHANG Xiang-hua; ZHANG Zhi-yi

The full-length cDNA library of male floral buds of Populus deltoides Marsh. was constructed throughSMATR ( the Switch Mechanism At the 5’end of RNA Temp lates) technique. The titer is larger than 106 pfu·mL-1, the percentage of recombination reaches 95%, and the average size of insert fragments is about 1kb. Theseresults indicate that the library is qualified for cloning and exp ressing target genes. The library has laid a foundationfor studies on the molecularmechanism of flower development in forest trees.

1. The Key Laboratory for Genetic and Breeding of Forest and Ornamental Plants,MOE. Beijing ForestryUniversity,Beijing　100083, China

2. Research Institute of Forestry, CAF

Received Date: 2005-09-06

Abstract: The full-length cDNA library of male floral buds of Populus deltoides Marsh. was constructed throughSMATR ( the Switch Mechanism At the 5’end of RNA Temp lates) technique. The titer is larger than 106 pfu·mL-1, the percentage of recombination reaches 95%, and the average size of insert fragments is about 1kb. Theseresults indicate that the library is qualified for cloning and exp ressing target genes. The library has laid a foundationfor studies on the molecularmechanism of flower development in forest trees.

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Reference (12)

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Construction of Full-length cDNA Library of MaleFloral Buds of Popu lus de ltoides

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