Micropropagation of Cym bidium hybridum by Inducementof PLB from Cuted Ba se of in vitro Plantlets
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1.
College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, J iangsu, China
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2.
Research Institute of Subtrop ical Forestry, CAF, Fuyang 311400, Zhejiang, China
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Received Date:
2007-02-05
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Abstract
PLB ( p rotocorm-like body ) is successfully induced from the cuted tissue of p lantlet2base of Cym bid iumhybridum in vitro, in a simulative manner like microtuber. The effects of different concentrations of 2, 4-D, 6-BA,and NAA on the PLB formation of C. hybridum were investigated in different level of concentration. The resultsshowed that the effects of 2, 4-D and 6-BA on the frequency of PLB formation of C. hybridum differed extremely significantly. 2, 4-D on lower levelwas a important stimulative to induce PLB, but the inducing of PLB would be restrained when the concentration of 2, 4-D was above 0. 60 mg·L-1. The frequency of PLB formation increased basically with the increase of concentrations of 6-BA. The frequency of PLB formation was also related to the genotype,NAA was a important stimulative factor for some special genotype of C. hybridum. The experiment strategy to inducing PLB in simulative manner of bulb is feasible.
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Proportional views
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