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Micropropagation of Cym bidium hybridum by Inducementof PLB from Cuted Ba se of in vitro Plantlets

  • Received Date: 2007-02-05
  • PLB ( p rotocorm-like body ) is successfully induced from the cuted tissue of p lantlet2base of Cym bid iumhybridum in vitro, in a simulative manner like microtuber. The effects of different concentrations of 2, 4-D, 6-BA,and NAA on the PLB formation of C. hybridum were investigated in different level of concentration. The resultsshowed that the effects of 2, 4-D and 6-BA on the frequency of PLB formation of C. hybridum differed extremely significantly. 2, 4-D on lower levelwas a important stimulative to induce PLB, but the inducing of PLB would be restrained when the concentration of 2, 4-D was above 0. 60 mg·L-1. The frequency of PLB formation increased basically with the increase of concentrations of 6-BA. The frequency of PLB formation was also related to the genotype,NAA was a important stimulative factor for some special genotype of C. hybridum. The experiment strategy to inducing PLB in simulative manner of bulb is feasible.
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    [2] 郑迎冬,杨承勇,蒋林. 大花蕙兰的茎段培养[J]. 仲恺农业技术学院学报, 2000, 13 (1) : 19~22

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    Nayak N R, Rath S P, Patnaik S. In vitro p ropagation of three ep iphytic orchids, Cym bidium aloifolium (L. ) Sw., Dendrobium aphyllum (Roxb. ) Fisch. and Dendrobium m oschatum (Buch-Ham) Sw.through thidiazuron-induced high frequency shoot p roliferation[J].Scientia Horticulturae, 1997, 71 (324) : 243~250
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Micropropagation of Cym bidium hybridum by Inducementof PLB from Cuted Ba se of in vitro Plantlets

  • 1. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, J iangsu, China
  • 2. Research Institute of Subtrop ical Forestry, CAF, Fuyang 311400, Zhejiang, China

Abstract: PLB ( p rotocorm-like body ) is successfully induced from the cuted tissue of p lantlet2base of Cym bid iumhybridum in vitro, in a simulative manner like microtuber. The effects of different concentrations of 2, 4-D, 6-BA,and NAA on the PLB formation of C. hybridum were investigated in different level of concentration. The resultsshowed that the effects of 2, 4-D and 6-BA on the frequency of PLB formation of C. hybridum differed extremely significantly. 2, 4-D on lower levelwas a important stimulative to induce PLB, but the inducing of PLB would be restrained when the concentration of 2, 4-D was above 0. 60 mg·L-1. The frequency of PLB formation increased basically with the increase of concentrations of 6-BA. The frequency of PLB formation was also related to the genotype,NAA was a important stimulative factor for some special genotype of C. hybridum. The experiment strategy to inducing PLB in simulative manner of bulb is feasible.

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