Construction of T－DNA Activation Tagging for Populus Functional Genomics

WANG Shu-guang; LUAN Wei-jiang; QIAO Gui-rong; SUN Zong-xiu; ZHUO Ren-ying

W ith the comp letion of the Populus genome sequence in 2006, the study on functional genomics in Populus has become a major task. Establishment of Populusmutant population is an essential app roach for forestry functional genomics study. The activation tagging is a p romising and powerful method to constructmutant library of Populus and to further discover new gene and elucidate its function now. A novel T-DNA activation tagging pCAS05was constructed for forest functional genomics in this study. We could select conveniently and easily transgenicp lants by sp raying Basta because bar gene is constructed in this vector as a selection marker. Moreover, we couldalso construct a large scale mutant library in Populus by this activation tagging because of effectively selection of bargene.

1. Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi, China

2. Research Institute of Subtrop ical Forestry, CAF, Fuyang　311400, Zhejiang, China

3. Research Institute of Subtrop ical Forestry, CAF, Fuyang　311400, Zhejiang, China

4. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou　310006, Zhejiang, China

Received Date: 2006-09-04

Abstract: W ith the comp letion of the Populus genome sequence in 2006, the study on functional genomics in Populus has become a major task. Establishment of Populusmutant population is an essential app roach for forestry functional genomics study. The activation tagging is a p romising and powerful method to constructmutant library of Populus and to further discover new gene and elucidate its function now. A novel T-DNA activation tagging pCAS05was constructed for forest functional genomics in this study. We could select conveniently and easily transgenicp lants by sp raying Basta because bar gene is constructed in this vector as a selection marker. Moreover, we couldalso construct a large scale mutant library in Populus by this activation tagging because of effectively selection of bargene.

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Reference (19)

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Construction of T－DNA Activation Tagging for Populus Functional Genomics

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