Effect of So ilWa ter Stress on Active OxygenMetabolism of Chestnut Seedling Leaves

SHI Zhong-jie; DU A-peng; HU Zhe-sen; XU Da-ping

Some indexes, such as the active oxygen p rotective system and some free radicles,were measured by thecontrolling the water in container seedlings. The results indicated that the total relative water contents and the p rotein contents dropped with the increase of stress intensity and the contents of total chlorophyll, chlorophyll a, chlorophyll b and carotinoid had a descending trend. The activities of the SOD, POD and CAT descended firstly, then ascended and descended lastly and the activity of ASP had a descending trend. The free radicles showed differentchanging trend: the O2 p roducing rates increased firstly, and then decreased and increased lately with the waterstress intensity, but the H2O2 contents raised firstly and then decreased. BothMDA content and membrane relativepermeability had an ascending trend with the increasing stress. The activity of the enzymes such as SOD, CAT andASP may evaluate the drought2resistant of the chestnut seedlings as indexes.

1. Research Institute of Trop ical Forestry, CAF, Guangzhou 510520, Guangdong, China

2. Research Institute of Forestry Ecology, Environmentand Protection, CAF, Beijing　100091, China

3. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China

Received Date: 2007-03-28

Abstract: Some indexes, such as the active oxygen p rotective system and some free radicles,were measured by thecontrolling the water in container seedlings. The results indicated that the total relative water contents and the p rotein contents dropped with the increase of stress intensity and the contents of total chlorophyll, chlorophyll a, chlorophyll b and carotinoid had a descending trend. The activities of the SOD, POD and CAT descended firstly, then ascended and descended lastly and the activity of ASP had a descending trend. The free radicles showed differentchanging trend: the O2 p roducing rates increased firstly, and then decreased and increased lately with the waterstress intensity, but the H2O2 contents raised firstly and then decreased. BothMDA content and membrane relativepermeability had an ascending trend with the increasing stress. The activity of the enzymes such as SOD, CAT andASP may evaluate the drought2resistant of the chestnut seedlings as indexes.

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Reference (23)

[1]	王爱国,罗广华. 羟自由基启动下的脱氧核糖降解及其产物的TBA反应[J]. 生物化学与生物物理进展, 1993, 20 ( 2 ) : 150～152
[2]	Arora A, Sairam R K, Srivastava G C. Oxidative stress and antioxidative system in p lants [J]. Current Science, 2002, 82: 1227～1238
[3]	叶冰莹,陈由强,朱锦懋,等. 水分胁迫对三种木麻黄小枝活性氧伤害的研究[J]. 福建师范大学学报(自然科学版) , 2000, 16(1) : 76～79
[4]	陈立松,刘星辉. 水分胁迫对荔枝叶片活性氧代谢的影响[J]. 园艺学报, 1998, 25 (3) : 241～246
[5]	时忠杰,胡哲森,李荣生. 水分胁迫与活性氧代谢[J] , 贵州大学学报(农业与生物科学版) , 2002, 21 (2) : 140～145
[6]	马双艳,姜远茂,彭福田,等. 干旱胁迫对不同板栗品种渗透调节物质含量及光合的影响[J]. 干旱地区农业研究, 2003, 21 ( 3) :114～118
[7]	于同泉,秦岭,陈静, 等. 水分胁迫对板栗幼苗抗氧化酶及丙二醛的影响[J]. 北京农学院学报, 1996, 11 (1) : 48～52
[8]	万善霞,秦岭,于同泉,等. 水分胁迫对板栗幼苗过氧化物酶、超氧物歧化酶活力及同工酶谱的影响[J]. 北京农学院学报, 1997,12 (3) : 20～24
[9]	华东师范大学生物系植物生理教研室. 植物生理学实验指导[M]. 北京:人民教育出版社, 1980: 1～3; 143～144
[10]	BrandfordM M. A rap id and sensitive method for the quantizationofmicrogram quantities of p rotein utilizing the p rincip le of p roteindye binding[J]. Annal Biochem, 1976, 72: 248～252
[11]	沈其伟. 测定水稻叶片叶绿素含量的混合液提取法[J]. 植物生理学通讯, 1988, 24 (3) : 62～64
[12]	邹琦. 植物生理生化实验指导[M]. 北京:中国农业出版社,1995: 70～72
[13]	Dhinsa R S, Dhinsa P P, Thorp T A. Leaf senescence correlatedwith increased levels ofmembrane permeability and lip id peroxidation and decreased levels of superoxide dismutase and catalase[J].Tour Exp Bot, 1980, 32: 93
[14]	张宪政. 作物生理研究法[M]. 北京:农业出版社, 1992: 208～209; 211～212; 210～211
[15]	X?H?波钦诺克(苏) . 植物生物化学分析方法[M]. 荆家海,丁钟荣译. 北京:科学出版社, 1981: 203～207; 209～212
[16]	林植芳,李双顺,林桂竹,等. 衰老叶片和叶绿体中H₂O₂的累积与膜脂过氧化的关系[J]. 植物生理学报, 1988, 14 (1) : 16～22
[17]	王爱国,罗广华. 植物的超氧物自由基与羟胺反应的定量关系[J]. 植物生理学通讯, 1990, 26 (6) : 55～57
[18]	刘宁,高玉葆,贾彩霞,等. 渗透胁迫下多花黑麦草叶内过氧化物酶活性和脯氨酸含量以及质膜相对透性变化[J]. 植物生理学通讯, 2000, 36 (1) : 11～15
[19]	王建华,刘鸿先,徐同. 超氧化物岐化酶( SOD)在植物逆境和衰老生理中的作用[J]. 植物生理学通讯, 1989, 25 (1) : 1～5
[20]	Scandalios J G. Oxygen stress and superoxide dismutasea [J].Plant Physiol, 1993, 101: 7～11
[21]	夏新莉,郑彩霞,尹伟伦. 土壤干旱对樟子松针叶膜脂过氧化膜脂成分和乙烯释放的影响[J]. 林业科学, 2000, 36 ( 3) : 8～12
[22]	魏炜,赵欣平,吕辉. 三种抗氧化酶在小麦抗干旱逆境中的作用初探[J]. 四川大学学报, 2003, 40 (6) : 1172～1175
[23]	Fridovich I. Superoxide dismutase [J] , Ann Rev Biochem, 1975,44: 147～159

Effect of So ilWa ter Stress on Active OxygenMetabolism of Chestnut Seedling Leaves

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References

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