Clon ing and Character iza tion of COM T Gene from B am busa oldham ii

LI Xue-ping; GAO Zhi-min; PENG Zhen-hua; YUE Yong-de; GAO Jian; CAI Chun-ju; MU Shao-hua

A gene of COM T family was cloned by RT-PCR and RACE from B am busa oldham ii and named B oCOM T1. B oCOM T1, with a full length of 1 377 bp, encoded a polypep tide of 361 amino acids with p redicted molecular mass of 39 kDa. The results of amino acid sequence analysis showed thatBoCOMT1 had the highest similarity of 86. 7% with TaCOMT, phylogenetic analysis showed that BoCOM T1 is more related to TaCOMT. The exp ression level of B oCOM T1 in stem is about two times as in leaf.

1. International Center for Bamboo and Rattan, Key Laboratory of Bamboo and Rattan Science and Technology,State Forestry Administration, Beijing　100102, China

2. Research Institute of Forestry, CAF, Beijing　100091, China

Received Date: 2007-04-09

Abstract: A gene of COM T family was cloned by RT-PCR and RACE from B am busa oldham ii and named B oCOM T1. B oCOM T1, with a full length of 1 377 bp, encoded a polypep tide of 361 amino acids with p redicted molecular mass of 39 kDa. The results of amino acid sequence analysis showed thatBoCOMT1 had the highest similarity of 86. 7% with TaCOMT, phylogenetic analysis showed that BoCOM T1 is more related to TaCOMT. The exp ression level of B oCOM T1 in stem is about two times as in leaf.

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Clon ing and Character iza tion of COM T Gene from B am busa oldham ii

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通讯作者: 陈斌, bchen63@163.com

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