Optim iza tion of Tissue Culture Conditions and Establishmentof Regenera tion System for Popu lus ×eu ram e ricana cl.‘J2’

ZHANG Lei; SU Xiao-hua; ZHANG Bing-yu; HUANG Qin-jun; ZHANG Xiang-hua; LI Yi-liang

The key factors affecting the growth of Populus ×euram ericana cl. ‘J2’in vitro and the op timal tissueculture conditions for it were ascertained. The high frequency regeneration system for P. ×euram ericana cl. ‘J2’was also established in this study. The op timum basic culture medium wasMS; the op timal subculture medium androot inducement culture medium for shootwere 1 /2MS + NAA 0. 08 mg·L-1 + IBA 0. 08·mg L-1 ; active carbon disadvantaged the growth of P. ×euram ericana cl. ‘J2’; photoperiod had little effect on P. ×eu ram ericana cl.‘J2’, while light intensity and the pH value of subculture medium had great effect on it, the op timal light intensitywas 2 500 lx and the op timal pH value was 6. 5; the op timal shoot inducement culture medium for leaf2exp lantwasMS + BA1. 0 mg·L-1 + NAA0. 1 mg·L-1 and for leafstalk-exp lantwasMS + BA 1. 0 mg·L-1 + NAA 0. 2 mg·L-1.

1. Forestry College, Shenyang Agriculture University, Shenyang　110161,Liaoning, China

2. Research Institute of Forestry, CAF

Received Date: 2006-09-07

Abstract: The key factors affecting the growth of Populus ×euram ericana cl. ‘J2’in vitro and the op timal tissueculture conditions for it were ascertained. The high frequency regeneration system for P. ×euram ericana cl. ‘J2’was also established in this study. The op timum basic culture medium wasMS; the op timal subculture medium androot inducement culture medium for shootwere 1 /2MS + NAA 0. 08 mg·L-1 + IBA 0. 08·mg L-1 ; active carbon disadvantaged the growth of P. ×euram ericana cl. ‘J2’; photoperiod had little effect on P. ×eu ram ericana cl.‘J2’, while light intensity and the pH value of subculture medium had great effect on it, the op timal light intensitywas 2 500 lx and the op timal pH value was 6. 5; the op timal shoot inducement culture medium for leaf2exp lantwasMS + BA1. 0 mg·L-1 + NAA0. 1 mg·L-1 and for leafstalk-exp lantwasMS + BA 1. 0 mg·L-1 + NAA 0. 2 mg·L-1.

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Reference (14)

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Optim iza tion of Tissue Culture Conditions and Establishmentof Regenera tion System for Popu lus ×eu ram e ricana cl.‘J2’

Abstract

References

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