Study on the Factors Affecting the Regeneration ofin vitro Leaves of Sophora japonica f. 'L iao Hong'

QIU Yan-chang; DUAN Zu-an; ZHANG Jin-fang; ZHANG Xian-guang; LIU Wei

The propagationa l mu ltiple o f Sophora japonica .f ‘L iao Hong’ tube seedlings w as less affected by theconcentration o f sucrose, but the concentration o f sucrose affected the grow th morpho logy o f tube seed lings. Whenthe sucrose concentration increased from 20 g? L-1 to 60 g? L-1 inWPM m edia, the leaves turned to norma l fromcrink led and became dark g reen. The procedures of leave regenerat ion cu lture are as fo llow s: The d ifferentiatedseedlings o fS. jap onica w ere inocu lated onWPM ( w ith 6- BA 2. 0mg? L-1 + NAA 0. 05mg? L-1 + sucrose 20~60 g? L-1 + agar 70 g? L-1 ) , after cu ltured for 20 days, the d ifferent iated tube seed lings w ere obta ined. Theleaves sheared w ere put into asepsis w aterw ith 1mg? L-1 V itam in C and soaked for 1~ 3m inutes, then planted ontoWPM ( w ith 6- BA 2. 0mg? L-1 + NAA 0. 1 mg? L-1 + sucrose 40 g? L-1 + agar 7. 0 g? L-1 ) , and culturedunder illum ination for 30~ 40 days, the differentiated cutt ing tissues w ere go.t Cultured under contro lled env ironment ( illum ination tim e 12 hours, dark culture for 12 hours, light intensity 2 000 lx, temperature 25 , dark cu lture tempera ture 15 , pH 5. 8) for 20~ 30 days, the green callus were induced; then they w ere transplan ted onWPM med ia (w ith 6- BA 4. 0mg? L-1 + NAA 0. 05mg? L-1 + GA 2mg? L-1 + sucrose 60 g? L-1 + agar 7?0 g? L-1 ) , the adventitious budsw ere germ inated from green callus t issues, and the regenerated adventitious buds ra tew as 89% .

1. L iaoch eng Un iversity of Shandong, L iaocheng?? 252000, Sh andong, Ch ina

2. Shandong A gricu ltu ralUn iversity, Tai' an?? 271000, Shandong, Ch ina

Received Date: 2007-06-28

Abstract: The propagationa l mu ltiple o f Sophora japonica .f ‘L iao Hong’ tube seedlings w as less affected by theconcentration o f sucrose, but the concentration o f sucrose affected the grow th morpho logy o f tube seed lings. Whenthe sucrose concentration increased from 20 g? L-1 to 60 g? L-1 inWPM m edia, the leaves turned to norma l fromcrink led and became dark g reen. The procedures of leave regenerat ion cu lture are as fo llow s: The d ifferentiatedseedlings o fS. jap onica w ere inocu lated onWPM ( w ith 6- BA 2. 0mg? L-1 + NAA 0. 05mg? L-1 + sucrose 20~60 g? L-1 + agar 70 g? L-1 ) , after cu ltured for 20 days, the d ifferent iated tube seed lings w ere obta ined. Theleaves sheared w ere put into asepsis w aterw ith 1mg? L-1 V itam in C and soaked for 1~ 3m inutes, then planted ontoWPM ( w ith 6- BA 2. 0mg? L-1 + NAA 0. 1 mg? L-1 + sucrose 40 g? L-1 + agar 7. 0 g? L-1 ) , and culturedunder illum ination for 30~ 40 days, the differentiated cutt ing tissues w ere go.t Cultured under contro lled env ironment ( illum ination tim e 12 hours, dark culture for 12 hours, light intensity 2 000 lx, temperature 25 , dark cu lture tempera ture 15 , pH 5. 8) for 20~ 30 days, the green callus were induced; then they w ere transplan ted onWPM med ia (w ith 6- BA 4. 0mg? L-1 + NAA 0. 05mg? L-1 + GA 2mg? L-1 + sucrose 60 g? L-1 + agar 7?0 g? L-1 ) , the adventitious budsw ere germ inated from green callus t issues, and the regenerated adventitious buds ra tew as 89% .

HTML

Reference (20)

[1]	赵燕, 侯桂玲, 张秀省, 等. 国槐及其变种、变型花粉形态的比较研究[J] . 聊城大学学报(自然科学版) , 2007, 20 ( 1) : 53 ~ 55
[2]	冷平生, 马世超, 李树蓉. 增施CO 气肥对国槐幼苗生长与生理特性的影响[J] . 林业科学, 2002, 38 ( 1) : 44 ~ 49
[3]	GevrenovaR, K itanovG, Ilieva D. On togenetic and season al variationin the f lavonoid composit ion ofS oph ora japonica cu ltivated in Bu lgaria[J] . Pharm aceut ica lB iology, 2007, 45 ( 2) : 149 ~ 155
[4]	魏红敏, 王燕军, 吴荣德. 国槐溃疡病的发生规律与防治措施[J] . 河南林业科技, 2003, 23 ( 2) : 52
[5]	闫家河, 李双云, 何邦今. 国槐新害虫—— 鸡血藤棕麦蛾生物学特性及防治[J]. 山东林业科技, 2007 ( 2) : 20 ~ 22
[6]	赵化奇, 桑景栓, 孙丹萍, 等. 锈色粒肩天牛卵空间分布型及抽样技术初步研究[J] . 植物保护, 2007 , 33( 1 ): 78~ 82
[7]	阎国华, 周宇, 张开春. 核果类果树转基因研究进展[J] . 果树科学, 2001, 18( 6 ) : 358~ 365
[8]	张晓英, 王华芳, 朱祯, 等. 国槐离体再生及抗虫基因sck 的转导[J] . 林业科学, 2006, 42( 9 ) : 34~ 38
[9]	Ito Y. O ccurren ce of lectin s in leaves and flow ers ofSophora japon ica[J] . Plan t Science, 1986, 47( 2 ) : 77 ~ 82
[10]	王桂兰, 陈超, 李伟, 等. 红掌叶片愈伤组织和气生根再生团块的细胞学研究[J]. 园艺学报, 2006, 33( 3) : 587~ 591
[11]	李文金, 贾汇红, 王均华, 等. 欧洲樱桃矮化砧木Rus－25 的组织培养与植株再生[J] . 植物生理学通讯, 2004, 40( 6 ) : 718~ 718
[12]	邱艳昌, 段祖安. 聊红槐微体快繁技术[J] . 林业实用技术, 2007( 7 ): 20~ 22
[13]	O zel C A, Arslan O. E fficien t m icrop ropagation of engl ish sh rubrose & h eritage? under in vi tro cond it ions [J] . International JournalofAgriculture and B io logy, 2006, 8 ( 5) : 626 ~ 629
[14]	孙清荣, 李勃, 张力思, 等. 樱桃品种吉列玛叶片再生不定梢的研究[J]. 落叶果树, 2001, 33( 1) : 10 ~ 11
[15]	丁世萍, 严菊强, 季道藩. 糖类在植物组织培养中的效应[J] . 植物学通报, 1998, 12( 6 ): 115~ 117
[16]	王玉华, 杨清, 陈敏, 等. 植物糖感知和糖信号传导[J] . 植物学通报, 2004, 21( 3 ) : 273~ 279
[17]	Tang Y P, Lou F C, W ang JH, et a.l Fou r new isoflavone triglycos ides from S ophora japonica [J]. Jou rnal ofN aturalProducts, 2001,64 ( 8) : 1107 ~ 1110
[18]	朱德蔚. 植物组织培养与脱毒快繁技术[M] . 北京: 中国科学技术出版社, 2001: 147 ~ 150
[19]	张晓英, 尹伟伦, 朱祯. 抗生素对国槐愈伤组织诱导和生长的影响[J] . 北京林业大学学报, 2004, 26( 6) : 62~ 65
[20]	M in B, Oh S R, Lee H K, et a.l Soph oricoside analogs as the IL－5inh ib itors from Sophora japonica [J]. Plan ta M ed ica, 1999, 65( 5) :408 ~ 412

Study on the Factors Affecting the Regeneration ofin vitro Leaves of Sophora japonica f. 'L iao Hong'

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views