Growth-promoting Effects and Physiolog ica l Property of Endophytic D iazotrophsIsola ted from Guangdong Bamboo ( B am busa blum eana )

HOU Wei; PENG Gui-xiang; XU Zhi-jun; CHEN Shi-xian; TAN Zhi-yuan

Five endophytic diazotrophs with high and stable nitrogenase activity screened from Guangdong bamboo(B am busa blum eana J. A. et J. H. Schult. f. )were studied by physiological test, the adap tability to environmentsand the growth-p romoting effects on rice. The results showed that these strains could grow well at 18—40 ℃and hadnitrogenase activity at 26—37 ℃for op timum growth under nitrogen-free medium. Strains also showed nitrogenaseactivity at a pH range of 5. 0 to 8. 0, at a saline (NaCl) concentration range of 0. 5—2. 5 g·L -1 and at a range of0. 05—0. 50 g·L -1 (NH4 ) 2 SO4. Strains can secrete acid or alkali substance as neutralizer at pH 8. 0 and 5. 0 respectively. Experiments of inoculation on rice showed that strains had obviously effects on p romoting the growth ofp lants.

1. Guangdong Provincial Key Lab of PlantMolecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642,Guangdong, China

2. College of Resources and Environment, South China Agricultural University, Guangzhou 510642, Guangdong, China

Received Date: 2005-10-11

Accepted Date: 2007-08-30

Abstract: Five endophytic diazotrophs with high and stable nitrogenase activity screened from Guangdong bamboo(B am busa blum eana J. A. et J. H. Schult. f. )were studied by physiological test, the adap tability to environmentsand the growth-p romoting effects on rice. The results showed that these strains could grow well at 18—40 ℃and hadnitrogenase activity at 26—37 ℃for op timum growth under nitrogen-free medium. Strains also showed nitrogenaseactivity at a pH range of 5. 0 to 8. 0, at a saline (NaCl) concentration range of 0. 5—2. 5 g·L -1 and at a range of0. 05—0. 50 g·L -1 (NH4 ) 2 SO4. Strains can secrete acid or alkali substance as neutralizer at pH 8. 0 and 5. 0 respectively. Experiments of inoculation on rice showed that strains had obviously effects on p romoting the growth ofp lants.

HTML

Reference (13)

[1]	Baldani J I, Olivares F L, Hemerly A S. Nitrogen-fixing endophytes: recent advances in the association with graminaceous p lantsgrown in the trop ics [M] / /Elemerich C, Kordorosi A, Newton WE. Biological Nitrogen Fixation for the 21 st Century. Netherlands:KluwerAcademic Publishers, 1998: 203 - 206
[2]	吕泽勋,李久蒂,朱至清. 玉米内生固氮菌的回接分离及限菌条件下在玉米根内的定殖[J]. 应用与环境生物学报, 2001, 7 (3) :207 - 212
[3]	Dêbereiner J. History and new perspectives of diazotrophs in association with non-leguminous p lants[J]. Symbiosis, 1993, 23: 1 - 13
[4]	Cojho E H, ReisVM, SchenbergA C G, et al. Interactions of Acetobacter diazotrophius with an amylolytic yeast in nitrogen-free bathculture[J]. FEMSMicrobiol lett, 1993, 106: 241 - 346
[5]	方　萍,高丽梅, 贾小明,等. 固氮螺菌(Azosprillum brasilense)NO40在红壤性水稻土上的接种效应[J]. 浙江大学学报:农业与生命科学版, 2001, 27 (1) : 33 - 36
[6]	侯　伟,彭桂香,许志钧,等. 广东省箣竹内生固氮菌多样性[J].农业生物技术学报, 2007, 15 (2) : 290 - 294
[7]	彭桂香,王华荣,侯　伟,等. 糖蜜草内生固氮菌IS2PCR和16SrRNA基因全序列分析[J]. 华南农业大学学报, 2005, 26 ( 4) :73 - 76
[8]	姚拓, 张德罡, 胡自治. 高寒地区燕麦根际联合固氮菌研究I.固氮菌分离及鉴定[J]. 草业学报, 2004, 13 (2) : 106 - 111
[9]	Yoshida S, Forno D A, Cock J H, et al. Laboratory Manual forPhysiological Studies of Rice. 3 rd Edition [M]. Manila, Philip-pines: IRR I, 1976: 61 - 64
[10]	张丽梅,方　萍,季天委. 高效固氮菌株W12 对环境因子的适应性研究[J]. 浙江大学学报: 农业与生命科学版, 2002, 28(6) : 664 - 668
[11]	Watanabe I, Lin C. Response of wetland rice to inoculation withAzospirillum liperferum and Pseudom onas sp. [J]. Soil Sci PlantNutr, 1984, 30: 117
[12]	李久蒂,安千里. 联合固氮研究进展[J]. 植物学通报, 1997, 14(3) : 14 - 21
[13]	顾小平,吴晓丽. 接种联合固氮菌对毛竹实生苗生长的影响[J]. 林业科学研究, 1999, 12 (1) : 7 - 12

Growth-promoting Effects and Physiolog ica l Property of Endophytic D iazotrophsIsola ted from Guangdong Bamboo ( B am busa blum eana )

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views