Study on the NaCl Concen tra tion and Subculture Times ofBluegra ss Sa lt-toleran t Ca llus Selection

LI Yin-feng; SUN Zhen-yuan; HAN Lei; LIU Cai-xia

The calluses induced from bluegrass seedswere treated with different concentrations of NaCl to test theirsurvival rate and quality. The results showed that the suitable concentration was 15 g·L -1 forNaCl-tolerant callusselecting. The analysis of survival rate and growth of callus showed that the most op timal subculture timeswere 6 forNaCl-tolerant callus induction of bluegrass under the suitable NaCl concentration. NaCl-tolerant stability test p rovedthat the gained bluegrass callus had steady NaCl-tolerance.

1. Research Institute of Forestry, CAF, Beijing　100091, China

Received Date: 2007-04-23

Abstract: The calluses induced from bluegrass seedswere treated with different concentrations of NaCl to test theirsurvival rate and quality. The results showed that the suitable concentration was 15 g·L -1 forNaCl-tolerant callusselecting. The analysis of survival rate and growth of callus showed that the most op timal subculture timeswere 6 forNaCl-tolerant callus induction of bluegrass under the suitable NaCl concentration. NaCl-tolerant stability test p rovedthat the gained bluegrass callus had steady NaCl-tolerance.

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Reference (10)

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Study on the NaCl Concen tra tion and Subculture Times ofBluegra ss Sa lt-toleran t Ca llus Selection

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