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Citation:

Pur if ica tion of Phenyla lan ine Ammon ia-lya se in Ca ragana korsh inskii Juven ile

  • Received Date: 2006-04-25
    Accepted Date: 2007-06-28
  • Phenylalanine ammonia-lyase ( PAL; EC 4. 3. 1. 5) of root in leaf stage, leaf and branch in rap id growthstage of Ca ragana korshinskii,was purified by ion exchange chromatography and gel filtration chromatography. Thesubunits of PAL by SDS2PAGE were analyzed. The results showed that when purified by ion exchangechromatography and gel filtration chromatography, there were two kinds of PAL , oneMW was 306KD and the otherwas 222KD, from leaf and one PAL whichMW was 222 KD from stem and root. By SDS-PAGE, it could concludethat the PAL, MW 306KD, was composed of two polypep tides which MW might be 82KD and two polypep tideswhich MW might 74 KD, and the 222KD-MW PAL was composed of four homology subunits, which MWwas 74KD.
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  • [1]

    Koukol J, Conn E E . The metablism of aromatic compounds in highp lants: Purification and p ropertics of the L-phenvlalanine deaminaseof Herdeum vulagare[J]. Biol Chem, 1961 (236) : 2692 - 2698
    [2] 牛西午,丁玉川,许 强,等. 营养液不同磷浓度对柠条苗期植株生长发育的影响[J]. 西北植物学报, 2003, 23 (4) : 622 - 627

    [3]

    Hahlbrock K, Ragg H. Light-induced changes of enzyme activities inparsley cell suspension cultures [J]. Archives of Biochemistry andBiophysics, 1975 , 166: 41 - 46
    [4]

    Koike M. Nanbu K. Phenylalanine ammonia2lyase activity in alfalfasuspension cultures treated with conidia and elicitors of Verticilliumalbo2atrum[J]. Biologia Plantarum, 1997 (5) : 349 - 353
    [5]

    Bradford M M. A rap id and sensitive method for the quantitation ofmicrogram quantities of p rotein utilizing the p rincip le of p rotein-dyebinding [J]. Analyt Biochem, 1976, 72: 248 - 254
    [6]

    Laemmli U K. Cleavage of structural p roteins during the assembly ofthe head of bacteriophage T4 [J]. Nature 1970, 227: 680 - 685
    [7] 方天纵,安守芹,赵怀青. 应用有序聚类法对柠条苗高生长期的划分[J]. 内蒙古林学院学报(自然科学版) , 1996, 18 (1) : 22 - 26

    [8] 江 柯,卢 涛,赵德利,等. 红酵母苯丙氨酸解氨酶的分离纯化及性质研究[J]. 四川大学学报(自然科学版) , 2004, 141(14) : 865 - 868

    [9] 刘卫红,王 燕,杜何为,等. 银杏叶苯丙氨酸解氨酶分离纯化条件的研究[J]. 湖北农学院学报, 2004, 24 (3) : 196 - 198

    [10]

    Bolwell G P,Bell J N, Cramer C L , et al. L2Phenylalanine ammonia2lyase from Phaseolus vulgaris characterisation and differential inductionof multiple forms from elicitor2treated cell suspension cultures [J].European Journal of Biochemistry, 1985, 149: 411 - 419
    [11]

    Bolwell G P, Cramer C L , Lamb C J, et al. L2Phenylalanine ammonia-lyase from Phaseolus vulgaris: partial degradation of enzymesubunits in vitro and in vivo[J]. Biochim Biophys Acta, 1986, 881:210 - 217
    [12]

    Bolwell G P, RodgersM W. L-phenylalanine ammonia-lyase fromFrench bean ( Phaseolus vulgarisL. ) characterization and differential exp ression of antigenic multip leMr forms[J]. Biochem J, 1991 ,279: 231 - 236
    [13]

    A da Cunha . Purification characterization and induction of L-phenylalanine ammonialyase in Phaseolus vulgaris[J] . European Journal of Biochemistry, 1988, 178: 243 - 248
    [14]

    Whetten RW , Sedevoff R R . Phenylalanine ammonia- lyase fromloblolly p ine [J]. Plant Physiology , 1992 , 98 (1) : 380 - 386
    [15]

    Lim HW , Park S S , Lim C J . Purification and p roperties of phenylalanine ammonia-lyase from leafmustard [J]. Molcell , 1997, 31;7 (6) : 715 - 720
    [16]

    K K Kalghatgi, P V Subba. Microbial L2phenylalanine ammonialyase. Purification, subunit structure and kinetic p roperties of theenzyme from Rhizoctonia solani[J]. Biochem J, 1975, 149: 65 - 75
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Pur if ica tion of Phenyla lan ine Ammon ia-lya se in Ca ragana korsh inskii Juven ile

  • 1. Institute of Animal Sciences, Chinese Academy ofAgricultural Sciences,Beijing 100094, China
  • 2. Graduate School, Chinese Academy of Agricultural Sciences,Beijing 100081, China

Abstract: Phenylalanine ammonia-lyase ( PAL; EC 4. 3. 1. 5) of root in leaf stage, leaf and branch in rap id growthstage of Ca ragana korshinskii,was purified by ion exchange chromatography and gel filtration chromatography. Thesubunits of PAL by SDS2PAGE were analyzed. The results showed that when purified by ion exchangechromatography and gel filtration chromatography, there were two kinds of PAL , oneMW was 306KD and the otherwas 222KD, from leaf and one PAL whichMW was 222 KD from stem and root. By SDS-PAGE, it could concludethat the PAL, MW 306KD, was composed of two polypep tides which MW might be 82KD and two polypep tideswhich MW might 74 KD, and the 222KD-MW PAL was composed of four homology subunits, which MWwas 74KD.

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